A polycistronic microRNA cluster, miR-17-92, is overexpressed in human lung cancers and enhances cell proliferation.

By Y. Hayashita, H. Osada, Y. Tatematsu, Hideki Yamada, K. Yanagisawa, S. Tomida, Yasushi Yatabe, K. Kawahara, Y. Sekido, Takashi Takahashi. Cancer Research, 2005

1,484 citations recorded by the source corpus.

MicroRNAs (miRNAs) are small noncoding RNAs, thought to be involved in physiologic and developmental processes by negatively regulating expression of target genes. We have previously reported frequent down-regulation of the let-7 miRNA family in lung cancers and, in the present study, assessed alteration in a panel of 19 lung cancer cell lines. As a result, we found for the first time that the miR-17-92 cluster, which comprises seven miRNAs and resides in intron 3 of the C13orf25 gene at 13q31.3, is markedly overexpressed in lung cancers, especially with small-cell lung cancer histology. Southern blot analysis revealed the presence of increased gene copy numbers of the miRNA cluster in a fraction of lung cancer cell lines with overexpression. In addition, we were able to show predominant localization of C13orf25 transcripts within the nucleus and introduction of the expression construct of the miR-17-92 cluster, but not the putative open reading frame of C13orf25, enhancing lung cancer cell growth. These findings clearly suggest that marked overexpression of the miR-17-92 cluster with occasional gene amplification may play a role in the development of lung cancers, especially in their most aggressive form, small-cell lung cancer, and that the C13orf25 gene may well be serving as a vehicle in this regard.

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/3cbf4d5de082d965ca33ca046da81a4e822c0a31

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Citations in the corpus

MicroRNA control in the immune system: basic principles. (2009, background): “…13q31, a genomic region that is frequently amplified in lymphomas and solid tissue cancers, and the mature miRNAs encoded by this locus are expressed in high amounts in those cancer cells (Hayashita et al., 2005; Lu et al., 2005; Ota et al., 2004; Tagawa and Seto, 2005; Zhang et al., 2007).”
A small piece in the cancer puzzle: microRNAs as tumor suppressors and oncogenes (2006, background): “These miRNAs are also widely overexpressed in an array of cancer types including lymphoma, colon, lung, breast, pancreas and prostate (Hayashita et al., 2005; He et al., 2005b; Volinia et al., 2006).”
microRNAs as oncogenes and tumor suppressors. (2007, background): “Compared with normal tissues, the expression of mir- 17–92 is significantly increased in several cancer types, including lung cancer and lymphomas, especially in their most aggressive forms, such as small-cell lung cancer and human B-cell lymphomas (Hayashita et al., 2005; He et al., 2005b).”
MicroRNAs in cell proliferation, cell death, and tumorigenesis (2006, background): “Amplification of the mir-17 cluster and overexpression of these miRNAs has more recently been observed in lung cancers and in particular small-cell lung cancer (Hayashita et al, 2005).”
MicroRNA signatures in human cancers (2006, methodology): “, and by Hayashita and colleagues and Tagawa and Seto using northern blot analyses in lung cancers and malignant lymphomas, respectivel”
MicroRNA function in cancer: oncogene or a tumor suppressor? (2009, background): “MiR-17-92 clusters including miR106a, miR17-5p, miR19a, miR-25, and miR-93 tend to be overexpressed in lung cancer tissues as compared to their expression in normal tissues [41].”
MicroRNAs: key players in the immune system, differentiation, tumorigenesis and cell death (2008, background): “In contrast to miR-15/miR-16, whose expression is often reduced in cancers, the miR-17-92 cluster is overexpressed in many cancers including B-cell lymphoma (Ota et al., 2004; Hayashita et al., 2005; He et al., 2005b).”
microRNAs in cancer (2013, background): “The miR-17/92 cluster is located within ∼1 kb of an intron of the C13orf25 locus on human chromosome 13q31, a region frequently amplified in several types of lymphoma and solid tumors (132, 133).”