Mammalian TOR complex 2 controls the actin cytoskeleton and is rapamycin insensitive

By E. Jacinto, R. Loewith, A. Schmidt, Shuo Lin, M. Rüegg, A. Hall, Michael N. Hall. Nature Cell Biology, 2004

2,219 citations recorded by the source corpus.

Subfields: Molecular Biology, Pharmacology

View Paper: https://www.semanticscholar.org/paper/3c7fde3d835e2c0ac29fe901bd7020705d8044bf

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Citations in the corpus

Insulin signalling to mTOR mediated by the Akt/PKB substrate PRAS40 (2007, background): “, or with rictor and GβL to form mTORC2, which is involved in cytoskeleton regulation and Akt phosphorylatio”
Active-Site Inhibitors of mTOR Target Rapamycin-Resistant Outputs of mTORC1 and mTORC2 (2009, background): “Previous analysis of mTORC2 function using RNAi revealed a role for mTORC2 in the control of the actin cytoskeleton [3,4], yet these findings were not confirmed in primary MEFs lacking mTORC2 [15,17].”
Defining the role of mTOR in cancer. (2007, background): “Following soon after was the unexpected identification of a second growth-factor-sensitive but nutrient-insensitive mTOR-containing complex called mTORC2 (Jacinto et al., 2004; Loewith et al., 2002; Sarbassov et al., 2004).”
SIN1/MIP1 maintains rictor-mTOR complex integrity and regulates Akt phosphorylation and substrate specificity. (2006, methodology): “Except for raptor immunoprecipitation, in which cells were lysed in buffer containing 0.3% CHAPS detergent, cells were lysed for coIP as described (Jacinto et al., 2004).”
mTOR Signaling in Growth, Metabolism, and Disease. (2017, background): “…of Raptor however, mTORC2 contains Rictor (rapamycin insensitive companion of mTOR), an unrelated protein that likely serves an analogous function (Jacinto et al. 2004; Sarbassov et al. 2004). mTORC2 also contains DEPTOR (Peterson et al. 2009), as well as the regulatory subunits mSin1 (Frias et…”
In brief (2011, neutral): “…in YPD at 25 C and then shifted to 37 C for 6 hr. Cell Culture, Immunoprecipitations, Immunoblotting, and mTORC2 Kinase Assay HeLa, melanoma cells, and colon cancer cells were cultured, transfected, stimulated, and harvested as described previously (Jacinto et al., 2004; Thedieck et al., 2007).”
Unraveling the Complex Interplay Between T Cell Metabolism and Function (2018, background): “In addition, Ras homolog gene family, member A (RhoA) signaling downstream of mTORC2 activation (104) is important for differentiation, but not maintenance, of Th2 cells (105).”