Refinement of Tools for Targeted Gene Expression in Drosophila

By B. Pfeiffer, Teri-Tb Ngo, Karen L. Hibbard, C. Murphy, Arnim Jenett, James W. Truman, Gerald M. Rubin. 2010

1,079 citations recorded by the source corpus.

Subfields: Cellular and Molecular Neuroscience, Genetics, Molecular Biology

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Citations in the corpus

Dopaminergic neurons write and update memories with cell-type-specific rules (2016, background): “Pairing one of two odors with activation of PPL1-g1pedc, results in robust aversive memory to the paired odor (Aso et al., 2010; Figure 1C–”
Mushroom body output neurons encode valence and guide memory-based action selection in Drosophila (2014, methodology): “pBDPGAL4U (attP2), an enhancerless GAL4 construct (Pfeiffer et al., 2010), was used as a control driver line in behavioral assays.”
The neuronal architecture of the mushroom body provides a logic for associative learning (2014, methodology): “…primers SM(1A) and BI(1S) (Alekseyenko et al., 2010) and cloned into pBPp65ADZpw and pBPZpGAL4DBDw using the Gateway system as previously described (Pfeiffer et al., 2008, 2010). pJFRC225-5xUAS-IVS-myr::smGFP-FLAG in VK00005 and pJFRC200-10xUAS-IVS-myr::smGFP-HA in attP18 are described by…”
Sleep facilitates memory by blocking dopamine neuron mediated forgetting (2015, neutral): “TH-lexA Construction To begin construction, we obtained nlsLexA:GADfl plasmid (26232, Addgene) (Pfeiffer et al., 2010) and pCaSpeR-TH (containing GAL4 encoding sequences) from Dr.”
Genetic manipulation of genes and cells in the nervous system of the fruit fly (2011, methodology): “…tdTomato-3xHA Membrane Red Potter et al., 2010 LexA 8xLexO CD2-GFP Membrane Green Lai and Lee, 2006 8x/13x/16x/26xLexO CD8-GFP Membrane Green Pfeiffer et al., 2010 8xLexO myr-mCherry Membrane Red Diegelmann et al., 2008 8xLexO mCherry-CAAX Membrane Red Yagi et al., 2010 Examples of…”
The VT GAL4, LexA, and split-GAL4 driver line collections for targeted expression in the Drosophila nervous system (2017, methodology): “We used vectors coding for LexA fusion proteins in which the LexA DNA-binding domain is coupled to either a GAL4 or a human p65 activation domain (Pfeiffer et al., 2010), thus providing both GAL80-suppressible and GAL80-insensitive LexA variants.”
Analysis of functional neuronal connectivity in the Drosophila brain. (2012, background): “…of specific drivers allows for many hypothesized connections between neuronal classes to be experimentally tested using the approach we have described, and the production of highly specific genetic drivers continues apace (e.g., Bohm et al. 2010; Luan and White 2007; Pfeiffer et al. 2008, 2010).”
Dopamine Neurons Mediate Learning and Forgetting through Bidirectional Modulation of a Memory Trace (2018, neutral): “, 2013); 10XUAS-IVS-myr::GFP in attP2 (Pfeiffer et al., 2010); 10XUAS-IVS-myr::tdTomato in attP2 (Pfeiffer et al.”
Heterosynaptic plasticity underlies aversive olfactory learning in Drosophila (2015, positive): “MB099C (TH-p65ADZp in VK00027, 73F07-ZpGAL4DBD in attP2) and MB320C (THp65ADZp in VK00027, 22B12-ZpGAL4DBD in attP2) were designed and constructed as previously described based onGAL4-line expression patterns (Aso et al., 2014a; Jenett et al., 2012; Pfeiffer et al., 2010).”
Using translational enhancers to increase transgene expression in Drosophila (2012, methodology): “Confirming our previous results (29), adding the WPRE to the 3′-UTR (Fig.”
Reward signal in a recurrent circuit drives appetitive long-term memory formation (2015, positive): “R72D01-LexA was constructed using the methods described in Pfeiffer et al. (2010) Ichinose et al. eLife 2015;4:e10719.”
A GAL4-driver line resource for Drosophila neurobiology. (2012, positive): “For example, GAL4 expression can be restricted to cells in which expression of two enhancer fragments overlap by using the split-GAL4 method, developed by Luan et al. (2006) and improved upon by Pfeiffer et al. (2010).”
Origins of Cell-Type-Specific Olfactory Processing in the Drosophila Mushroom Body Circuit. (2017, positive): “…1996), UAS-ReaChR::Citrine (attP40) (Inagaki et al., 2014), UAS-mCD8::GFP (attP40) (Lee and Luo, 1999), UAS-myr::GFP (attP40), UAS-myr::GFP (attP2) (Pfeiffer et al., 2010), UAS-CsChrimson::mVenus (attP40) (Klapoetke et al., 2014), UAS-GCaMP5G (attP40) (Akerboom et al., 2012), UAS-GCaMP6s (attP40)…”
A Complete Electron Microscopy Volume of the Brain of Adult Drosophila melanogaster (2017, background): “…(e.g. Hige et al., 2015; Wilson et al., 2004), relatively small size (Figure 1A), and the stereotypy of and genetic access to most of the ~100,000 neurons in its brain (Aso et al., 2014; Chiang et al., 2011; Jenett et al., 2012; Kvon et al., 2014; Milyaev et al., 2012; Pfeiffer et al., 2010).”