Chronic Peroxisome Proliferator-activated Receptor γ (PPARγ) Activation of Epididymally Derived White Adipocyte Cultures Reveals a Population of Thermogenically Competent, UCP1-containing Adipocytes…

By N. Petrovic, Tomas B. Waldén, I. Shabalina, J. Timmons, B. Cannon, J. Nedergaard. Journal of Biological Chemistry, 2009

1,287 citations recorded by the source corpus.

The recent insight that brown adipocytes and muscle cells share a common origin and in this respect are distinct from white adipocytes has spurred questions concerning the origin and molecular characteristics of the UCP1-expressing cells observed in classic white adipose tissue depots under certain physiological or pharmacological conditions. Examining precursors from the purest white adipose tissue depot (epididymal), we report here that chronic treatment with the peroxisome proliferator-activated receptor γ agonist rosiglitazone promotes not only the expression of PGC-1α and mitochondriogenesis in these cells but also a norepinephrine-augmentable UCP1 gene expression in a significant subset of the cells, providing these cells with a genuine thermogenic capacity. However, although functional thermogenic genes are expressed, the cells are devoid of transcripts for the novel transcription factors now associated with classic brown adipocytes (Zic1, Lhx8, Meox2, and characteristically PRDM16) or for myocyte-associated genes (myogenin and myomirs (muscle-specific microRNAs)) and retain white fat characteristics such as Hoxc9 expression. Co-culture experiments verify that the UCP1-expressing cells are not proliferating classic brown adipocytes (adipomyocytes), and these cells therefore constitute a subset of adipocytes (“brite” adipocytes) with a developmental origin and molecular characteristics distinguishing them as a separate class of cells.

Subfields: Epidemiology, Physiology, Rehabilitation

View Paper: https://www.semanticscholar.org/paper/39a185288b4ac5bd6c682582584a57b95eae3568

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Epidemiology

1. S. Kajimura
2. B. Spiegelman
3. B. Cannon
4. H. Ohno
5. Jun Wu
6. Kosaku Shinoda
7. Toren Finkel
8. E. Rosen

In Physiology

1. S. Kajimura
2. B. Spiegelman
3. Jun Wu
4. J. Nedergaard
5. B. Cannon
6. Kosaku Shinoda
7. H. Ohno
8. Toren Finkel

In Rehabilitation

1. S. Kajimura
2. B. Spiegelman
3. Kosaku Shinoda
4. J. Nedergaard
5. H. Ohno
6. B. Cannon
7. P. Seale

Citations in the corpus

• Human BAT Possesses Molecular Signatures That Resemble Beige/Brite Cells (2012, background): “This is consistent with the previous studies by Cannon and Nedergaard’s group [19,20].”
• Brown and beige fat in humans: thermogenic adipocytes that control energy and glucose homeostasis. (2015, neutral): “Cellular identity of adult human BAT — is human BAT recruitable? Recent studies have identified several genes that are preferentially expressed either in classical brown adipocytes or in beige adipocytes in rodents (26, 39, 45, 46).”
• In vivo identification of bipotential adipocyte progenitors recruited by β3-adrenoceptor activation and high fat feeding (2012, positive): “Several groups have isolated distinct cell populations from adipose tissues and muscle that have the potential to form white or brown adipocytes in vitro and in vivo, following transplantation (Joe et al., 2010; Petrovic et al., 2010; Rodeheffer et al., 2008; Schulz et al., 2011; Uezumi et al., 2010”
• Irisin Stimulates Browning of White Adipocytes Through Mitogen-Activated Protein Kinase p38 MAP Kinase and ERK MAP Kinase Signaling (2014, background): “These brown fat cells, UCP-1 positive, are designated as beige or brown-in-white (brite) cells (11,34).”
• Adaptive thermogenesis in adipocytes: is beige the new brown? (2013, neutral): “Chronic treatment of primary precursors from the visceral WAT depot (epididymal) with PPARg agonists showed that only a subpopulation of these precursors can attain a ‘‘brown’’-like phenotype (Petrovic et al. 2010).”
• UCP1 in brite/beige adipose tissue mitochondria is functionally thermogenic. (2013, methodology): “The ‘‘white’’ adipose tissues and cells that display the ability to express UCP1 are presently mainly referred to as brite (Petrovic et al., 2010) or beige (Ishibashi and Seale, 2010); we use here the brite nomenclature.”
• Nonshivering thermogenesis and its adequate measurement in metabolic studies (2011, background): “the appearance of UCP1-containing cells in white adipose tissue depots (Xue et al., 2005; Petrovic et al., 2010; Waldén, 2010)].”