UCP1 in brite/beige adipose tissue mitochondria is functionally thermogenic.

By I. Shabalina, N. Petrovic, Jasper M. A. de Jong, A. Kalinovich, B. Cannon, J. Nedergaard. Cell Reports, 2013

611 citations recorded by the source corpus.

The phenomenon of white fat "browning," in which certain white adipose tissue depots significantly increase gene expression for the uncoupling protein UCP1 and thus supposedly acquire thermogenic, fat-burning properties, has attracted considerable attention. Because the mRNA increases are from very low initial levels, the metabolic relevance of the change is unclear: is the UCP1 protein thermogenically competent in these brite/beige-fat mitochondria? We found that, in mitochondria isolated from the inguinal "white" adipose depot of cold-acclimated mice, UCP1 protein levels almost reached those in brown-fat mitochondria. The UCP1 was thermogenically functional, in that these mitochondria exhibited UCP1-dependent thermogenesis with lipid or carbohydrate substrates with canonical guanosine diphosphate (GDP) sensitivity and loss of thermogenesis in UCP1 knockout (KO) mice. Obesogenic mouse strains had a lower thermogenic potential than obesity-resistant strains. The thermogenic density (UCP1-dependent oxygen consumption per g tissue) of inguinal white adipose tissue was maximally one-fifth of interscapular brown adipose tissue, and the total quantitative contribution of all inguinal mitochondria was maximally one-third of all interscapular brown-fat mitochondria, indicating that the classical brown adipose tissue depots would still predominate in thermogenesis.

Subfields: Cardiology and Cardiovascular Medicine, Cell Biology, Physiology

View Paper: https://www.semanticscholar.org/paper/3f5a01a4786311bf4d0e4c4eddaa892117c4a6a6

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Cardiology and Cardiovascular Medicine

1. R. Zechner
2. B. Spiegelman

In Cell Biology

1. E. Wagner
2. P. Seale
3. B. Spiegelman
4. S. Kajimura

In Physiology

1. P. Seale
2. S. Kajimura
3. B. Spiegelman
4. R. Zechner
5. E. Rosen
6. L. Sidossis
7. R. Scarpulla
8. Christoph Handschin

Citations in the corpus

• Brown and beige fat: Physiological roles beyond heat-generation (2015, background): “Notably, fully stimulated beige adipocytes express similar UCP1 levels as brown adipocytes and undergo UCP1-mediated uncoupled respiration (Long et al., 2014; Okamatsu-Ogura et al., 2013; Shabalina et al., 2013; Wu et al., 2012).”
• A switch from white to brown fat increases energy expenditure in cancer-associated cachexia. (2014, background): “WAT browning is responsible for a significant increase in total energy expenditure (Shabalina et al., 2013), and stimulation of browning has therapeutic potential to promote body fat reduction (Yoneshiro et al., 2013).”
• Brown and beige fat in humans: thermogenic adipocytes that control energy and glucose homeostasis. (2015, neutral): “When stimulated by such external cues, beige adipocytes express UCP1 protein at a similar level to classical brown adipocytes and exhibit UCP1-dependent thermogenic capacity (25, 26).”
• Intermittent Fasting Promotes White Adipose Browning and Decreases Obesity by Shaping the Gut Microbiota (2017, neutral): “…or EODF/MT (EODF versus AL or EODF/MT versus AL/MT drunk with the same water), and red letters show the effects of microbiota depletion (AB versus CV within the same feeding regimen). promote thermogenesis and suppresses obesity (Ishibashi and Seale, 2010; Shabalina et al., 2013; Wu et al., 2012).”
• Mitochondrial electron transport chain, ROS generation and uncoupling (Review) (2019, background): “UcP1 can also be detected in the beige adipocytes of white adipose tissue (WAT) during thermal acclimation under specific conditions (152).”