Germline Transmission and Tissue-Specific Expression of Transgenes Delivered by Lentiviral Vectors

By C. Lois, Elizabeth J. Hong, S. Pease, E. Brown, David Baltimore. 2002

2,098 citations recorded by the source corpus.

Subfields: Genetics, Molecular Biology, Computational Biology & Bioinformatics

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Citations in the corpus

Predicting multicellular function through multi-layer tissue networks (2017, background): “In particular, the precise functions of proteins frequently depend on the tissue, and different proteins can have different cellular functions in different tissues (Lois et al., 2002; Rakyan et al., 2008; Magger et al., 2012; Guan et al., 2012; Fagerberg et al., 2014; Yeger-Lotem and Sharan, 2015; H”
Tie2 identifies a hematopoietic lineage of proangiogenic monocytes required for tumor vessel formation and a mesenchymal population of pericyte progenitors. (2005, methodology): “We injected the LV into the perivitelline space of fertilized oocytes, as described (Lois et al., 2002 and the Supplemental Experimental Procedures in the Supplemental Data available with this article online) and selected mouse lines carrying multiple vector copies per genome to amplify detection…”
Conditional mouse lung cancer models using adenoviral or lentiviral delivery of Cre recombinase (2009, background): “2 (gag/pol) (plasmid 8455) and transfer vector expressing Cre (modified from plasmid 17408)) ( Addgene”
In vivo fate mapping and expression analysis reveals molecular hallmarks of prospectively isolated adult neural stem cells. (2010, methodology): “, 2008) and NCre behind the human GFAP promoter (hGFAP-NCre) into a FUGW lentiviral backbone (Lois et al., 2002) to achieve expression of functional CCre::NCre dimers only in cells expressing from both promoters hGFAP and P2.”
The timing of cortical neurogenesis is encoded within lineages of individual progenitor cells (2006, methodology): “The shRNA Foxg1 cassette was cloned into a self-inactivating lentiviral vector derived from FUGW”
Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells. (2008, methodology): “Viral Constructs To generate the tetracycline-inducible viral backbone, the human ubiquitin C promoter and intron were excised from FUW (Lois et al., 2002) by first digesting with PacI, followed by 30 overhang removal with Klenow and subsequent digest with BamHI.”
Young dentate granule cells mediate pattern separation, whereas old granule cells facilitate pattern completion. (2012, neutral): “…were unexpected given that previous theoretical and experimental work has suggested that the EC/ DG/CA3 pathway is crucial for pattern separation (Marr, 1971; McNaughton and Morris, 1987; Treves and Rolls, 1994; Gilbert et al., 2001; Leutgeb et al., 2007; McHugh et al., 2007; Hunsaker and…”
Origin and progeny of reactive gliosis: A source of multipotent cells in the injured brain (2008, neutral): “7) generated as described (28) or by transient cotransfection of 293T cells with pFUGW (41), where eGFP is encoded under the control of the human ubiquitin C promoter, the packaging plasmid pCMV R8.”
Mitochondrial Fusion Protects against Neurodegeneration in the Cerebellum (2007, neutral): “Retroviral stocks were produced as described (Lois et al., 2002), except that viral supernatants were collected in cerebellar culture media.”
c-Myc is dispensable for direct reprogramming of mouse fibroblasts. (2008, background): “After infection with a GFP-expressing lentivirus (FUW-GFP) (Lois et al., 2002) and injection into diploid blastocysts, the three-factor iPS cells generated live newborn (F and F0) and adult chimeric mice (G).”
Dicer-deficient mouse embryonic stem cells are defective in differentiation and centromeric silencing. (2005, methodology): “Lentiviruses were packaged and pseudotyped with the vesicular stomatitis virus glycoprotein (VSV-G) as previously described (Lois et al. 2002).”