Identification of a novel family of oligodendrocyte lineage-specific basic helix-loop-helix transcription factors.

By Qiao Zhou, Songli Wang, David J. Anderson. Neuron, 2000

806 citations recorded by the source corpus.

Basic helix-loop-helix (bHLH) transcription factors have been identified for neurons and their precursors but not for glial cells. We have identified two bHLH factors, Oligo1 and Oligo2, that are specifically expressed in zones of neuroepithelium from which oligodendrocyte precursors emerge, as well as in the precursors themselves. Expression of Oligo2 in the spinal cord precedes that of platelet-derived growth factor receptor alpha (PDGFRalpha), the earliest known marker of oligodendrocyte precursors, by several days. Ectopic expression of Oligo2 in vivo causes ectopic expression of Sox10, an HMG-box transcription factor expressed in oligodendrocyte and other glial precursors. These data identify Oligo genes as the earliest known markers of oligodendrocyte lineage determination and suggest they play a causal role in this process.

Subfields: Developmental Neuroscience, Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/27901a447ad019398f8bbc3926605e3016afe02f

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Citations in the corpus

The bHLH transcription factor Olig2 promotes oligodendrocyte differentiation in collaboration with Nkx2.2. (2001, methodology): “2 cells appear to delaminate and migrate2000; Xu et al., 2000; Zhou et al., 2000; Soula et al., 2001), we sought to compare their expression more directly.”
Multi-compartment microscopic diffusion imaging (2016, background): “Both models are expected to impact myelination in the central nervous system, as Olig2 plays an important role in the formation of oligodendrocyte precursors and oligodendrocyte differentiation (Lu et al., 2000; Zhou et al., 2000).”
Timing of CNS cell generation: a programmed sequence of neuron and glial cell production from isolated murine cortical stem cells. (2000, background): “…helix-loop-helix proteins oligo-1 and oligo-2, which are initiated early in oligodendrocyte formation,stem cells may well be applicable to the behavior of stem cells in other regions of the developing CNS, such relates to the switch point (Lu et al., 2000; Zhou et al., 2000).as the spinal cord.”
The basic helix-loop-helix transcription factor family in plants: a genome-wide study of protein structure and functional diversity. (2003, neutral): “E12 and related proteins are also involved in the determination and maintenance of other cell types through their ability to dimerize with other proteins that are expressed in specific cell lineages (Littlewood and Evan 1998, pp. 27-35).”
The Age-Related Decrease in CNS Remyelination Efficiency Is Attributable to an Impairment of Both Oligodendrocyte Progenitor Recruitment and Differentiation (2002, background): “To provide additional evidence that OPs were depleted from the area of demyelination, we also looked for expression of Olig-1 mRNA, an oligodendrocyte lineage transcription factor expressed immediately before PDGF- R mRNA during development, but unlike PDGF- R, also expressed at subsequent stages of”
The bHLH transcription factors OLIG2 and OLIG1 couple neuronal and glial subtype specification. (2002, neutral): “In heterozygotes, Olig2-hGFP -derived precursors gave is restricted to oligodendrocyte precursors (Lu et al., 2000; Zhou et al., 2000), nearly all OLIG2-positive cells rise to Islet1/2 (cid:2) motoneurons (Figure 3A, yellow cells) but not Chx10 (cid:2) V2 interneurons (Figure 3B).”
Coordinate regulation of motor neuron subtype identity and pan-neuronal properties by the bHLH repressor Olig2. (2001, background): “1999; Pierani et al., 1999; Briscoe et al., 2000; Zhou et al., 2000, 2001; Anderson, D.J., and Jan, Y.N. (1997).”
Human embryonic stem cells differentiate into oligodendrocytes in high purity and myelinate after spinal cord transplantation (2005, background): “The Olig1 and Olig2 transcription factors are critical for oligodendrocyte and motor neuron specification (Lu et al., 2000; Takebayashi et al., 2000; Zhou et al., 2000), and SOX10, a member of the highmobility group domain family of DNA binding proteins, is expressed within oligodendrocyte…”
The repair Schwann cell and its function in regenerating nerves (2016, background): “Other genes including Olig1, Shh and artemin are absent/low both in immature cells and in earlier stages of the Schwann cell lineage (Lu et al. 2000; Zhou et al. 2000; Lin et al. 2015).”
Origin of Oligodendrocytes in the Subventricular Zone of the Adult Brain (2006, methodology): “By using light and electron microscopy, we confirmed that Olig2, a transcription factor that plays an essential role in the generation of oligodendrocytes during embryogenesis (Zhou et al., 2000; Lu et al., 2002), is expressed in a small subpopulation of type C cells (Aguirre et al.”
Terminal differentiation of myelin-forming oligodendrocytes depends on the transcription factor Sox10. (2002, background): “In the CNS, Sox10 is one of the few transcription factors restricted to the oligodendrocyte lineage (Lu et al. 2000; Xu et al. 2000; Zhou et al. 2000).”
Common developmental requirement for Olig function indicates a motor neuron/oligodendrocyte connection. (2002, positive): “Olig genes mark oligodendrocyte precursors and their progeny in a restricted domain of the murine ventral spinal Results cord (Lu et al., 2000; Zhou et al., 2000).”
Neurogenin promotes neurogenesis and inhibits glial differentiation by independent mechanisms. (2001, neutral): “We have uncovered a novel mechanism of neurogenin action that does not rely on neurogenin’s These findings suggest that endogenous neurogenins and related bHLH proteins regulate the timing of glial production in the in vivo cortex.”
The Sox9 transcription factor determines glial fate choice in the developing spinal cord. (2003, background): “Ectopic expression of Olig2 has previously been shown to induce oligodendrocyte fate selectively in mesenchymally derived tissues of the chick embryo (Zhou et al. 2000).”