Development and validation of an improved oxygen radical absorbance capacity assay using fluorescein as the fluorescent probe.

By B. Ou, Maureen Hampsch-Woodill, R. Prior. Journal of Agricultural and Food Chemistry, 2001

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Review of Methods to Determine Antioxidant Capacities (2009, methodology): “ORAC measures antioxidant inhibition of peroxyl-radicalinduced oxidations and reflects classical radical chainbreaking antioxidant activity by H-atom transfer (Ou et al. 2001).”
Effects of environmental factors on dimethylated sulfur compounds and their potential role in the antioxidant system of the coral holobiont (2014, methodology): “AOC and protein content—Samples for AOC and protein concentrations were analyzed using the oxygen radical absorbance capacity (ORAC) assay (Ou et al. 2001) and Biuret assay (Dustin 1950), respectively, as described in Deschaseaux et al.”
Fluorescence probes used for detection of reactive oxygen species. (2005, methodology): “Because of this there have been used other fluorescent indicators such as fluorescein [130] and one of its derivatives, 6-carboxyfluorescein [128].”
Comparative evaluation of various total antioxidant capacity assays applied to phenolic compounds with the CUPRAC assay. (2007, methodology): “ORAC reaction, β-PE, showed inconsistent reactivity toward ROO from lot-to-lot, was photobleached due to reasons other than peroxyl radical attack, and exhibited non-specific protein binding to condensed tannins, therefore the probe of the ORAC test was later changed to fluorescein, though reporting”