Engineering a mevalonate pathway in Escherichia coli for production of terpenoids

By V. Martin, D. Pitera, Sydnor T. Withers, J. Newman, Jay D. Keasling. Nature Biotechnology, 2003

1,721 citations recorded by the source corpus.

Subfields: Molecular Biology, Pharmacology

View Paper: https://www.semanticscholar.org/paper/141e76be66fc755778332dc9f1723fe2fe9eb235

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Citations in the corpus

Engineering Cellular Metabolism (2008, neutral): “Often, expression of a heterologous metabolic pathway elicits a stress response in the host, due to protein overproduction or accumulation of toxic intermediates or end products (Gill et al., 2000; Martin et al., 2003).”
Isoprene Production Via the Mevalonic Acid Pathway in Escherichia coli (Bacteria) (2012, background): “Enhancing cellular metabolic flux toward DMAPP is a strategy to improve rates and yield of isoprenoid production by fermentative microbes [20, 21].”
Genetic Engineering of Algae for Enhanced Biofuel Production (2010, background): “coli, resulting in production of 112 mg/liter isopentenol (107, 200).”
Metabolic Engineering of Terpenoid Biosynthesis in Plants (2006, background): “They are commercially important due to their wide application in a vast number of industrial products such as flavouring agents, pharmaceuticals, perfumes, insecticides and anti-microbial agents (Martin et al., 2003).”
Foundations for engineering biology (2005, background): “The success of the building process depends on (1) the existence of a limited set of predefined, refined materials that can be delivered on demand and that behave as expected, (2) generally useful rules (that is, simple models) that describe how materials can be used in combination (or not), and (3)”
Paper-based Synthetic Gene Networks (2014, neutral): “In doing so, synthetic biologists have created whole-cell biosensors (Kobayashi et al., 2004; Kotula et al., 2014), synthetic probiotics, new sources of drugs (Fossati et al., 2014), green energy, (Torella et al., 2013) and chemistry (Zhang et al., 2012; Martin et al., 2003).”
Production of amorphadiene in yeast, and its conversion to dihydroartemisinic acid, precursor to the antimalarial agent artemisinin (2012, background): “The use of the galactoseregulated GAL1,10 promoters to overexpress the mevalonate pathway genes allowed repression of expression during strain construction, thus avoiding buildup of any potentially toxic intermediates (8) which may have resulted in strain instability during construction.”
Synthetic protein scaffolds provide modular control over metabolic flux (2009, methodology): “We selected the three-enzyme pathway that produces mevalonate from acetyl-CoA as a model syste”
Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System (2015, background): “Metabolic engineering is widely applied to modify Escherichia coli to produce industrially relevant biofuels or biochemicals, including ethanol (1), higher alcohols (2), fatty acids (3), amino acids (4), shikimate precursors (5), terpenoids (6), polyketides (7), and polymeric precursors of 1,4-butan”