Metabolic Engineering of Terpenoid Biosynthesis in Plants
By A. Aharoni, M. Jongsma, Tok-Yong Kim, Manalo Ri, A. Giri, F. Verstappen, W. Schwab, H. Bouwmeester. Phytochemistry Reviews, 2006
173 citations recorded by the source corpus.
Metabolic engineering of terpenoids in plants is a fascinating research topic from two main perspectives. On the one hand, the various biological activities of these compounds make their engineering a new tool for improving a considerable number of traits in crops. These include for example enhanced disease resistance, weed control by producing allelopathic compounds, better pest management, production of medicinal compounds, increased value of ornamentals and fruit and improved pollination. On the other hand, the same plants altered in the profile of terpenoids and their precursor pools make a most important contribution to fundamental studies on terpenoid biosynthesis and its regulation. In this review we describe our recent results with terpenoid engineering, focusing on two terpenoid classes the monoterpenoids and sesquiterpenoids. The emerging picture is that engineering of these compounds and their derivatives in plant cells is feasible, although with some requirements and limitations. For example, in terpenoid engineering experiments crucial factors are the subcellular localisation of both the precursor pool and the introduced enzymes, the activity of endogenous plant enzymes which modify the introduced terpenoid skeleton, the costs of engineering in terms of effects on other pathways sharing the same precursor pool and the phytotoxicity of the introduced terpenoids. Finally, we will show that transgenic plants altered in their terpenoid profile exert novel biological activities on their environment, for example influencing insect behaviour.
Subfields: Biochemistry, Biotechnology, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/a1cd7115843a1aee60fd302fc534b004c7f5420e
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Citations in the corpus
- Engineering secondary metabolite production in hairy roots (2011, background): “…a large number of traits in different crop species like enhanced disease resistance, weed control by producing allelopathic compounds, improved pest control, increased value of ornamentals and fruit (fragrance and flavor) and improved pollination by altering scent profiles (Aharoni et al. 2006).”
- Biosynthesis of essential oils in aromatic plants: A review (2016, background): “plant defense and the function of some essential oil components in plant-insect interactions, yet in transgenic Arabidopsis, the conversion of carbon to produce linalool due to overexpression of FaNES1 led to a phenotype having retarded growth, which was inherited through several generations of tran”
- Variation in natural plant products and the attraction of bodyguards involved in indirect plant defense (2010, background): “The genomic toolbox provides different options to manipulate a plant’s genotype to specifically change the plant in the desired phenotypic trait, including gene silencing and gene overexpression (Gurr and Rushton 2005a, 2005b; Aharoni et al. 2006).”