Transforming single DNA molecules into fluorescent magnetic particles for detection and enumeration of genetic variations

By D. Dressman, Hainen Yan, Giovanni Traverso, Kenneth W. Kinzler, Bert Vogelstein. Proceedings of the National Academy of Sciences of the United States of America, 2003

830 citations recorded by the source corpus.

Subfields: Cancer Research, Ecology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/13242a5e70ecfd9e4db557e86d16645b72175847

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Cancer Research

In Ecology

In Molecular Biology

Citations in the corpus

Fluorouracil, leucovorin, and irinotecan plus cetuximab treatment and RAS mutations in colorectal cancer. (2015, methodology): “The method selected to screen for RAS mutations was the BEAMing technique.(9) Although this approach can detect KRAS exon 2 mutations at a level of 0.”
Durable Remissions with Ivosidenib in IDH1‐Mutated Relapsed or Refractory AML (2018, neutral): “Second or later relapse 20 (16) 26 (15) —”
Utilization of next-generation sequencing platforms in plant genomics and genetic variant discovery (2010, methodology): “…from the two previous platforms in that it uses a sequencing-by-ligation approach (Shendure et al. 2005) to sequence tens to hundreds of millions of adapter-flanked DNA fragments which are immobilized on the surface of 1 lM paramagnetic beads and amplified via emulsion PCR (Dressman et al. 2003).”
DNA sequencing at 40: past, present and future (2017, neutral): “Clinical applications (NIPT) such that copies of each template are immobilized on beads that are then arrayed on a surface for sequencing 40–42 .”
Next-generation sequencing platforms. (2013, background): “Amplification of library fragments occurs by a unique approach known as emulsion PCR, which quantitates the library fragments and dilutes them to be mixed in equimolar quantities with small beads, PCR reactants, and DNA polymerase molecules (32).”
Target-enrichment strategies for next-generation sequencing (2010, neutral): “The technology uses microdroplets, similarly to emulsion PCR 20,21 .”
Phase II Pilot Study of Vemurafenib in Patients With Metastatic BRAF-Mutated Colorectal Cancer (2015, methodology): “15% on FFPE tissue specimens (Data Supplement).(15) A complementary droplet digital PCR was applied to a second cohort of 19 BRAF mutant tumors from patients not treated on the protocol but obtained from the Royal Melbourne Hospital (Parkville, Victoria, Australia).”
Sequencing technologies — the next generation (2010, methodology): “The two most common methods are emulsion PCR (emPCR”
Advanced sequencing technologies: methods and goals (2004, neutral): “The Vogelstein group recently developed a fourth method for achieving clonal amplification, known as BEAM (for beads, emulsion, amplification, magnetic”
Next generation sequencing technology: Advances and applications. (2014, methodology): “For the PGM/Proton sequence platforms the sequence templates are generated on a bead or sphere via emulsion PCR (emPCR) [5,6].”
The impact of next-generation sequencing technology on genetics. (2008, background): “oration by DNA polymerase to fuel a downstream set of reactions that ultimately produces light from the cleavage of oxyluciferin by luciferase [3] (Figure 1). Instead of sequencing in discrete tubes or in microtiter plate wells, the DNA strands of the library are amplified en masse by emulsion PCR [”