Target-enrichment strategies for next-generation sequencing
By Lira Mamanova, A. Coffey, C. Scott, I. Kozarewa, Emily H. Turner, Akash Kumar, E. Howard, Jay Shendure, D. Turner. Nature Methods, 2010
643 citations recorded by the source corpus.
Subfields: Molecular Biology
View Paper: https://www.semanticscholar.org/paper/ab05e334a8fa18763e11cf567886a4195932ffa0
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Molecular Biology
Citations in the corpus
- A review of the trade in orchids and its implications for conservation (2018, positive): “Finding suitable markers for species distinction is facilitated by innovations in high-throughput sequencing approaches that provide vastly more data for selection of variable markers, such as gene-capture and target-enrichment sequencing, genome skimming and Hyb-Seq (Mamanova et al., 2010).”
- Applications of next generation sequencing in molecular ecology of non-model organisms (2011, methodology): “Targets for sequencing can be obtained either using PCR or genetic capture techniques before sequencing (Mamanova et al., 2010).”
- Cost-effective, high-throughput DNA sequencing libraries for multiplexed target capture (2012, background): “An important feature of our libraries compared with almost all other Illumina library preparation methods (Cummings et al. 2010; Mamanova et al. 2010; Meyer and Kircher 2010; Teer et al. 2010) is that we add a 6-bp ‘‘internal’’ barcoded adapter to each fragment (Craig et al. 2008).”
- High-Throughput Genomic Data in Systematics and Phylogenetics (2013, methodology): “Advances in DNA sequencing technologies have been accompanied by a proliferation of methods for genomic partitioning, which are techniques for enriching sequence libraries for specific regions of a genome (also termed target enrichment; Turner et al. 2009, Mamanova et al. 2010).”