Next-generation sequencing platforms.

By Elaine R. Mardis. Annual Review of Analytical Chemistry, 2013

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The sooty moulds (2014, background): “The major difference of NGS to other molecular technologies is that taxa or Operational Taxonomic Units (OTUs) are no longer separated prior to sequencing by cultivation or subcloning of PCR products (mostly Intergenic Transcribed Spacer region ribosomal RNA or ITS rRNA gene amplicons), but the mole”
The Next-Generation Sequencing Revolution and Its Impact on Genomics (2013, neutral): “The use of multiplex primer pairs couples the high throughput of NGS platforms and the fact that each sequence read represents a single DNA product in the mixture due to the nature of the sequencing platforms (Mardis, 2013).”
Methods for the extraction, storage, amplification and sequencing of DNA from environmental samples (2018, background): “Uptake of metagenomics in recent years has increased substantially, and is continuing to gain in popularity, buoyed by improvements in sequencing yields and lengths (Mardis 2013), alongside the development of analysis tools and expertise.”