Marked differences in differentiation propensity among human embryonic stem cell lines

By K. Osafune, L. Caron, M. Borowiak, Rita J. Martinez, Claire Fitz-Gerald, Yasunori Sato, Chad A. Cowan, K. Chien, D. Melton. Nature Biotechnology, 2008

807 citations recorded by the source corpus.

Subfields: Molecular Biology, Surgery

View Paper: https://www.semanticscholar.org/paper/12efeef23cf326bd392b086d5c0058d537b31531

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

1. G. Daley
2. R. Jaenisch
3. Daisy A. Robinton
4. Stuart H. Orkin
5. Rafael A. Irizarry
6. J. Hanna
7. Martin J. Aryee
8. Andrew P. Feinberg

In Surgery

1. D. Melton

Citations in the corpus

• Somatic cell reprogramming and transitions between pluripotent states: facts, hypotheses, unresolved issues (2010, neutral): “Conventional human ESCs are impractical for use in diseaserelated research because of the laborious culture conditions required for their maintenance, their low efficiencies of genetargeting by homologous recombination, and the dramatic heterogeneity in differentiation potential among different huma”
• The promise of induced pluripotent stem cells in research and therapy (2012, background): “It is also important to consider that, in contrast to long-standing belief, ES cells themselves have considerable epigenetic heterogeneity and have differing propensities for differentiation — much like those found in iPS cell”
• Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2 (2008, background): “Two factor–induced iPS cells were successfully differentiated into neurons (ectoderm derivative) co-expressing βIII-tubulin and TH (tyrosine hydroxylase), the latter being a marker for dopaminergic neurons, beating cardiomyocytes (mesoderm derivative) and definitive endoderm as well as endoderm-deri”
• Induced pluripotent stem cells: past, present, and future. (2012, neutral): “Starting with the seminal studies by the groups led by George Daley (Park et al., 2008a), and Kevin Eggan (Dimos et al., 2008), more than 100 reports published in the past three years use diseasespecific iPSCs.”
• Reference Maps of Human ES and iPS Cell Variation Enable High-Throughput Characterization of Pluripotent Cell Lines (2011, neutral): “For example, HUES8 showed the greatest propensity for endoderm differentiation, corroborating previous results showing that this cell line performs well in directed endoderm differentiation (Osafune et al., 2008).”
• Neural differentiation of human induced pluripotent stem cells follows developmental principles but with variable potency (2010, background): “Various hESC lines differentiate into NE at a similar efficiencyunderour chemically definedadherent colony culture (29), thus minimizing the propensities that may manifest if using alternative protocols (30, 31).”
• Immunogenicity of induced pluripotent stem cells. (2011, neutral): “ESCs themselves have been shown to have a wide range of diversity in their differentiation potential.(5) A study using additional ESC lines would be necessary to prove that the immunogenicity observed in this article is specific for iPSCs.”
• Epigenetic memory in induced pluripotent stem cells (2010, background): “Human ESC can also manifest variable differentiation potential 47 .”
• Nephron organoids derived from human pluripotent stem cells model kidney development and injury (2015, neutral): “Intrinsic differences between HDF-α hiPSCs and H9 ESCs 28,29 mandated slight modifications to the protocol to optimize the production of posterior IM cells.”