Efficient differentiation of human embryonic stem cells to definitive endoderm

By K. D’Amour, A. Agulnick, Susan Eliazer, Olivia G. Kelly, Evert J Kroon, E. Baetge. Nature Biotechnology, 2005

1,734 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/12614efd99ebad91ff0ce7bc42823f6808a36296

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Citations in the corpus

Stoichiometric and temporal requirements of Oct4, Sox2, Klf4, and c-Myc expression for efficient human iPSC induction and differentiation (2009, methodology): “Endoderm and mesoderm were induced as previously described (37, 38).”
Production of pancreatic hormone–expressing endocrine cells from human embryonic stem cells (2006, methodology): “Previously we described a method for differentiating hES cells to D”
Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling (2009, background): “, suppression of mesodermal and endodermal fates by inhibiting endogenous activin and BMP signal”
Generation of functional human pancreatic β cells in vitro (2014, background): “…for the pancreatic lineage, and these have been effectively used to form cells in the b cell lineage in vitro from hPSC. Definitive endoderm and subsequent pancreatic progenitors can now be differentiated with high efficiencies (Kroon et al., 2008; D’Amour et al., 2005, 2006; Rezania et al., 2012).”
Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2 (2008, background): “Two factor–induced iPS cells were successfully differentiated into neurons (ectoderm derivative) co-expressing βIII-tubulin and TH (tyrosine hydroxylase), the latter being a marker for dopaminergic neurons, beating cardiomyocytes (mesoderm derivative) and definitive endoderm as well as endoderm-deri”
SOX17 is a Critical Specifier of Human Primordial Germ Cell Fate (2014, background): “This was unexpected because the primary role of SOX17 is in the endoderm (D’Amour et al., 2005; Kanai-Azuma et al., 2002) and because Sox17 has no detectable role in the specification of mouse PGCs (Hara et al.”
In vitro generation of human pluripotent stem cell derived lung organoids (2015, background): “Several reports have demonstrated that directed differentiation of human pluripotent stem cells (hPSCs), which include embryonic (hESCs) and induced (iPSCs) stem cells, is one of the most efficient approaches to achieving differentiation of a cell or tissue of interest (D’Amour et al., 2005; Kroon…”
Type 1 diabetes: etiology, immunology, and therapeutic strategies. (2011, background): “For instance, Viacyte differentiates hESCs into pancreatic endoderm and has plans to subsequently encapsulate these cells in a protective permeable device for transplantation (Encaptra, see website) (106, 234).”
Efficient generation of transgene-free human induced pluripotent stem cells (iPSCs) by temperature-sensitive Sendai virus vectors (2011, methodology): “1 mM ascorbic acid and 20% FBS to enhance cardiomyocyte differentiation (25), whereas an established protocol for hESCs involving activin A treatment (26) was used for the induction of definitive endoderm cells.”