Generation of functional human pancreatic β cells in vitro
By F. Pagliuca, J. Millman, Mads Gürtler, Michael Segel, Alana Van Dervort, J. Ryu, Quinn P. Peterson, D. Greiner, D. Melton. Cell, 2014
1,865 citations recorded by the source corpus.
Summary The generation of insulin-producing pancreatic β cells from stem cells in vitro would provide an unprecedented cell source for drug discovery and cell transplantation therapy in diabetes. However, insulin-producing cells previously generated from human pluripotent stem cells (hPSC) lack many functional characteristics of bona fide β cells. Here we report a scalable differentiation protocol that can generate hundreds of millions of glucose-responsive β cells from hPSC in vitro. These stem cell derived β cells (SC-β) express markers found in mature β cells, flux Ca2+ in response to glucose, package insulin into secretory granules and secrete quantities of insulin comparable to adult β cells in response to multiple sequential glucose challenges in vitro. Furthermore, these cells secrete human insulin into the serum of mice shortly after transplantation in a glucose-regulated manner, and transplantation of these cells ameliorates hyperglycemia in diabetic mice.
Subfields: Molecular Biology, Surgery
View Paper: https://www.semanticscholar.org/paper/cf413b70011c922426f880152c64247899d98d05
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In Molecular Biology
- Allon M. Klein
- Samuel L. Wolock
- D. Melton
- Renaud Gaujoux
- Itai Yanai
- Fabian J Theis
- S. Yamanaka
- Sarah A. Teichmann
In Surgery
Citations in the corpus
- A Single-Cell Transcriptomic Map of the Human and Mouse Pancreas Reveals Inter- and Intra-cell Population Structure (2016, positive): “In particular, significant efforts have been made to produce insulin-secreting beta cells in vitro from pluripotent cells (Nostro et al., 2015; Pagliuca et al., 2014; Rezania et al., 2014; Russ et al., 2015; Xie et al., 2013).”
- Biology-inspired Microphysiological System Approaches to Solve the Prediction Dilemma of Substance Testing (2016, neutral): “, 2013), pancreas (Pagliuca et al., 2014), kidney (Song et al.”