ATP-Driven Exchange of Histone H2AZ Variant Catalyzed by SWR1 Chromatin Remodeling Complex

By G. Mizuguchi, Xuetong Shen, Joseph Landry, Wei-Hua Wu, S. Sen, Carl Wu. Science, 2004

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The conserved histone variant H2AZ has an important role in the regulation of gene expression and the establishment of a buffer to the spread of silent heterochromatin. How histone variants such as H2AZ are incorporated into nucleosomes has been obscure. We have found that Swr1, a Swi2/Snf2-related adenosine triphosphatase, is the catalytic core of a multisubunit, histone-variant exchanger that efficiently replaces conventional histone H2A with histone H2AZ in nucleosome arrays. Swr1 is required for the deposition of histone H2AZ at specific chromosome locations in vivo, and Swr1 and H2AZ commonly regulate a subset of yeast genes. These findings define a previously unknown role for the adenosine triphosphate–dependent chromatin remodeling machinery.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/0e045753580a44eb6108d2161d2f6e7821b8aeee

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Citations in the corpus

The biology of chromatin remodeling complexes. (2009, background): “Remarkably, all nuclear ARPs are components of SWI/SNF and INO80 family chromatin remodeling complexes (Table 1) (8, 112, 145, 146).”
A Protein Complex Containing the Conserved Swi2/Snf2-Related ATPase Swr1p Deposits Histone Variant H2A.Z into Euchromatin (2004, background): “Z deposition (Krogan et al. 2003; Mizuguchi et al. 2004).”
The role of chromatin during transcription. (2007, background): “Z can be deposited into a nucleosome either through ATP-dependent histone exchange reactions (Mizuguchi et al., 2004) or with the help of a replication-independent chaperone Nap1 (Park et al., 2005).”
Recruitment of the INO80 complex by H2A phosphorylation links ATP-dependent chromatin remodeling with DNA double-strand break repair. (2004, background): “In ino80 cells, we find 1156 genes (i.e., 647 more than reported in Mizuguchi et al., 2004) either induced or repressed by at least 1.5-fold (Supplemental (induced or repressed) by at least 1.5-fold (Figures 1C and 1D), and among these genes the vast majority respond indistinguishably in wild-type,…”
Genome-wide dynamics of Htz1, a histone H2A variant that poises repressed/basal promoters for activation through histone loss. (2005, positive): “First, chromatin can bear nucleosomes formed from the four canonical histones (H2A, H2B, H3, and H4,) or nucleosomes bearing histone variants that help specialize chromatin regions (Henikoff et al., 2000; Henikoff et al., 2004; Mizuguchi et al., 2004; Smith, 2002).”
An essential switch in subunit composition of a chromatin remodeling complex during neural development. (2007, background): “BAF complexes contain ten core subunits, some of which are similar to those in yeast SWI/SNF, while others, such as BAF57 and BAF53, are similar to subunits of the yeast SWR1 and INO80 complexes (Mizuguchi et al., 2004; Shen et al., 2003).”
Dynamic regulation of nucleosome positioning in the human genome. (2008, background): “Z by the ATP-dependent Ino80 into nucleosomes (Kobor et al., 2004; Krogan et al., 2003; Mizuguchi et al., 2004) may also facilitate nucleosome eviction or repositioning by destabilizing the nucleosome structure (Luger et al., 1997).”
The X and Y Chromosomes Assemble into H2A.Z, Containing Facultative Heterochromatin, following Meiosis (2006, background): “chromatin loci (31, 32, 42), is required for telomeric silencing”
H2A.Z-containing nucleosomes mediate the thermosensory response in Arabidopsis. (2010, positive): “Z into nucleosomes in place of H2A (Deal et al., 2007; Kobor et al., 2004; Krogan et al., 2003; Mizuguchi et al., 2004).”