Activation of the Raf-MEK-ERK pathway is required for neutrophil extracellular trap formation.

By Abdul Hakkim, T. Fuchs, N. E. Martinez, S. Hess, H. Prinz, Arturo Zychlinsky, H. Waldmann. Nature Chemical Biology, 2011

758 citations recorded by the source corpus.

Subfields: Immunology, Immunology and Allergy, Physiology

View Paper: https://www.semanticscholar.org/paper/0df95c82bf8d877f6abea4da8a162fa81d20cc51

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Immunology

1. P. Kubes
2. B. Yipp
3. V. Brinkmann
4. Arturo Zychlinsky
5. Zena Werb
6. N. Borregaard
7. V. Papayannopoulos
8. K. Martinod

In Immunology and Allergy

1. Zena Werb
2. Arturo Zychlinsky
3. P. Kubes

In Physiology

1. V. Brinkmann
2. P. Kubes
3. Arturo Zychlinsky

Citations in the corpus

• Diverse stimuli engage different neutrophil extracellular trap pathways (2017, background): “DOI: 10.7554/eLife.24437 1 of 21 activate protein kinase C (PKC), which in turn leads to calcium fluxes within the cell and activation of the NADPH oxidase signalling cascade resulting in the production of reactive oxygen species (ROS) (Hakkim et al., 2011; Kaplan and Radic, 2012).”
• NETosis: how vital is it? (2013, background): “As previously detailed, suicidal NETosis requires PMA stimulation of raf-MEK-ERK and subsequent activation of NADPH oxidase.(7) MPO and elastase mediate the decondensation of chromatin resulting in a mixture of DNA and granule proteins that are extruded out of a perforation in the plasma membrane.”
• Inhibition of PAD4 activity is sufficient to disrupt mouse and human NET formation (2015, neutral): “14), using a custom-designed computer algorithm to quantify distinct NETotic morphologies 19 .”
• SK3 channel and mitochondrial ROS mediate NADPH oxidase-independent NETosis induced by calcium influx (2015, background): “ERK has been implicated as the major kinase involved in NOXdependent NETosis (14, 16).”
• Neutrophil extracellular traps: Is immunity the second function of chromatin? (2012, methodology): “Downstream of PKC but up­ stream of PHOX, the NET signaling cascade includes the Raf– MEK–ERK kinase pathway (Hakkim et al., 2011) and Rac2 (Ras­related C3 botulinum toxin substrate 2; Lim et al.”
• Gasdermin D plays a vital role in the generation of neutrophil extracellular traps (2018, background): “ROS signaling releases a macromolecular complex called azurosome from granules, which translocates neutrophil elastase (NE) to the nucleus (17, 18).”
• Neutrophils: Between Host Defence, Immune Modulation, and Tissue Injury (2015, background): “In addition to ROS, several other pathways have been reported to be involved in NET formation, such as peptidyl arginine deiminase (PAD) 4 [64], the Raf-MEK (mitogen-activated kinase/ERK kinase)-ERK (extracellular signalregulated kinase) pathway [65] and autophagy [66].”
• Platelets induce neutrophil extracellular traps in transfusion-related acute lung injury. (2012, background): “The canonical Raf/MEK/ERK signaling pathway is essential for PMA-induced NET formation (26).”
• Neutrophil Extracellular Traps Directly Induce Epithelial and Endothelial Cell Death: A Predominant Role of Histones (2012, background): “PMA, at this concentration, does not promote apoptosis or necrosis; rather, it induces typical features of NETosis [4,40–44].”