Specific Aquaporins Facilitate the Diffusion of Hydrogen Peroxide across Membranes*

By G. Bienert, A. Møller, K. Kristiansen, A. Schulz, I. Møller, J. Schjoerring, T. Jahn. Journal of Biological Chemistry, 2007

1,253 citations recorded by the source corpus.

The metabolism of aerobic organisms continuously produces reactive oxygen species. Although potentially toxic, these compounds also function in signaling. One important feature of signaling compounds is their ability to move between different compartments, e.g. to cross membranes. Here we present evidence that aquaporins can channel hydrogen peroxide (H2O2). Twenty-four aquaporins from plants and mammals were screened in five yeast strains differing in sensitivity toward oxidative stress. Expression of human AQP8 and plant Arabidopsis TIP1;1 and TIP1;2 in yeast decreased growth and survival in the presence of H2O2. Further evidence for aquaporin-mediated H2O2 diffusion was obtained by a fluorescence assay with intact yeast cells using an intracellular reactive oxygen species-sensitive fluorescent dye. Application of silver ions (Ag+), which block aquaporin-mediated water diffusion in a fast kinetics swelling assay, also reversed both the aquaporin-dependent growth repression and the H2O2-induced fluorescence. Our results present the first molecular genetic evidence for the diffusion of H2O2 through specific members of the aquaporin family.

Subfields: Biomedical Engineering, Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/0d7b5df088f9a131edd720ffe81c8df3c6f50e54

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Citations in the corpus

Signal transduction by reactive oxygen species (2011, background): “The source of this dysregulated oxidant production appears to be the mitochondria (Anderson et al., 2009), and by unknown mechanisms, high glucose conditions can cause alterations in mitochondrial morphology including the rapid fragmentation of mitochondria (Yu et al., 2006; Bonnard et al., 2008).”
Aquaporin-3 mediates hydrogen peroxide uptake to regulate downstream intracellular signaling (2010, background): “More recent studies implicate the role of AQP water channels, a class of membrane-spanning proteins that facilitate the diffusion of water and other substrates with varying specificity (26–28), in mediating H2O2 passage across the plasma membrane of reconstituted yeast (29) and plant (30) cells.”
Comprehending crystalline β-carotene accumulation by comparing engineered cell models and the natural carotenoid-rich system of citrus (2011, positive): “On the other hand, apoplastic H2O2 can traverse the plasma membrane (Moller and Sweetlove, 2010) into the cytosol via plasma membrane-localized aquaporins (Bienert et al., 2007; Miller et al., 2010).”
Reactive oxygen species (ROS) and response of antioxidants as ROS-scavengers during environmental stress in plants (2014, background): “It can cross membranes via aquaporins and cover considerable lengths within the cell (Bienert et al., 2007) and cause oxidative damage.”
Oxidative Stress, Prooxidants, and Antioxidants: The Interplay (2014, background): “2 readily diffuses across biological membranes, and so it is well-suited as a diffusible messenger [85, 86].”
Multiple functional roles of anthocyanins in plant-environment interactions. (2015, background): “The rapid movement of H2O2 into the vacuole is indeed facilitated by aquaporins, which are commonly involved in H2O transport (Bienert et al., 2007; Maurel et al., 2009).”
Chemistry and biology of reactive oxygen species in signaling or stress responses (2011, neutral): “Building on previous work in plant and yeast model”
Hydrogen Peroxide and Plant Stress: A Challenging Relationship (2007, background): “from the apoplast to the cytosol) probably involve controlled passage through aquaporins (Bienert et al. 2007).”
Plant aquaporins: membrane channels with multiple integrated functions. (2008, background): “Finally, expression in yeast cells deficient in endogenous systems responsible for urea or hydrogen peroxide uptake has proved efficient to screen, on the basis of a survival assay, aquaporin isoforms that possibly transport these molecules; these properties are subsequently confirmed by true transp”
Reactive oxygen species in cancer (2010, background): “Recent data suggest that hydrogen peroxide may cross cellular membranes through specific members of the aquaporin family [7].”
Functional and molecular characteristics of rice and barley NIP aquaporins transporting water, hydrogen peroxide and arsenite (2014, positive): “…were used in this study; the ΔSKN7 strain that has an impaired oxidative stress defense response and is sensitive to H2O2 (Bienert et al. 2007) and the ΔACR3 strain that lacks an arsenite extrusion transporter (Maciaszczyk-Dziubinska et al. 2010) and is sensitive to arsenite (Bienert et al. 2007).”
Oxidative modifications to cellular components in plants. (2007, background): “Its relative stability and ability to cross membranes possibly through aquaporins (9, 10) makes H2O2 a good messenger molecule.”
Reactive Oxygen Species in Inflammation and Tissue Injury (2014, positive): “H2O2, on the other hand, can cross biological membranes through aquaporin channels such as AQP3 and AQP8 which mediate membrane H2O2 uptake, raising the possibility that it can enter cells that are contacting one another (37, 165, 290).”
The Impact of Oxidative Stress and Inflammation on RPE Degeneration in Non-neovascular AMD (2017, background): “For example, oxidant-producing enzymes are located adjacent to the intended target, or oxidants have a regulated entry, such as H2O2 through aquaporin channels (Bienert et al., 2007).”