Production and characterization of clinical grade exosomes derived from dendritic cells.

By H. Lamparski, Anita Metha-Damani, Jenq-Yuan Yao, Sanjay Patel, D. Hsu, C. Ruegg, J. Le Pecq. JIM - Journal of Immunological Methods, 2002

682 citations recorded by the source corpus.

Subfields: Immunology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/0c0acfe7d9575f970fd434895c09deba81a45212

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Citations in the corpus

High Levels of Exosomes Expressing CD63 and Caveolin-1 in Plasma of Melanoma Patients (2009, background): “. These structures are secreted under specific physiological conditions from different cell types such as dendritic cells (DC), lymphocytes, mast cells and epithelial cells”
Exosome Theranostics: Biology and Translational Medicine (2018, background): “These techniques are not always mutually exclusive, and their combinations may be valuable [64].”
Standardization of sample collection, isolation and analysis methods in extracellular vesicle research (2013, neutral): “(171,224) or iodixanol (71) before centrifugation for a few hours to overnight.”
Dendritic cell-derived exosomes as maintenance immunotherapy after first line chemotherapy in NSCLC (2015, methodology): “Hence, over the last decade, Dex have been developed as clinical cell-free cancer vaccines.(7,8)”
Membrane vesicles as conveyors of immune responses (2009, methodology): “These trials required the establishment of good manufacturing procedures to obtain clinical-grade exosomes from patients' DC”
Biogenesis of extracellular vesicles (EV): exosomes, microvesicles, retrovirus-like vesicles, and apoptotic bodies (2013, neutral): “Other methods of isolation include: (1) the use of serial filtration [18], and (2) immune-isolation employing magnetic beads conjugated with anti-bodies directed specifically at proteins that are overrepresented on EVs [19, 20].”
Using exosomes, naturally-equipped nanocarriers, for drug delivery (2015, methodology): “For example, culturing DCs for extended time period [29], or at low pH [120] increased the release of exosomes 5–10 fold.”
Review of the Isolation, Characterization, Biological Function, and Multifarious Therapeutic Approaches of Exosomes (2019, background): “Subcutaneous/intradermal injections of DEX lead to the stabilization of disease or an objective tumor response [275].”
Exosomes in Cancer Development, Metastasis and Drug Resistance: A Comprehensive Review (2013, positive): “The above proof-of-concept studies paved the way for the production, isolation, and characterization of clinical grade exosomes derived from dendritic cells [204].”
Exosomes: extracellular organelles important in intercellular communication. (2010, methodology): “Two Phase 1 clinical trials for melanoma and lung cancer, using peptide antigens [67,68], were completed using this purification strategy.”