Molecular Diversity of Lactobacillus spp. and Other Lactic Acid Bacteria in the Human Intestine as Determined by Specific Amplification of 16S Ribosomal DNA

By H. Heilig, Erwin G. Zoetendal, E. Vaughan, P. Marteau, A. Akkermans, W. D. de Vos. Applied and Environmental Microbiology, 2002

730 citations recorded by the source corpus.

Subfields: Food Science, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/09005cfa360bf3c11b8fb63a483365eed9595be1

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Citations in the corpus

Mucosa-Associated Bacteria in the Human Gastrointestinal Tract Are Uniformly Distributed along the Colon and Differ from the Community Recovered from Feces (2002, methodology): “After purification with the Qiaquick PCR purification kit (Qiagen, Hilden, Germany), the Lactobacillus group-specific PCR was performed with primers Bact-0124-GCf and Lab-0677r followed by DGGE analysis on 8% polyacrylamide gels containing a urea-formamide gradient from 30 to 60% ( 8 ).”
Diversity and Succession of the Intestinal Bacterial Community of the Maturing Broiler Chicken (2003, background): “We identified several 16S rDNA sequences from chickens with identity to gastrointestinal microflora sequences from other animal species, including humans (22, 43, 58).”
Genes and Molecules of Lactobacilli Supporting Probiotic Action (2008, background): “sakei can also be found in the human GIT at fluctuating levels (104, 267).”
Assessment of novel probiotic Lactobacillus casei strains for the production of functional dairy foods (2004, methodology): “The detection of Lactobacillus-like bacteria in faecal samples was performed by PCR using the selective primers SD-Bact-0011-aS17 and SG-Lab-0677-aA17 (Heilig et al., 2002).”
Development of an extensive set of 16S rDNA‐targeted primers for quantification of pathogenic and indigenous bacteria in faecal samples by real‐time PCR (2004, background): “…with rRNA-targeted probes (Doré et al. 1998; Sghir et al. 2000; Marteau et al. 2001), denaturing gradient gel electrophoresis (Satokari et al. 2001; Heilig et al. 2002), temperature gradient gel electrophoresis (Zoetendal et al. 1998), and cloning and sequencing of rDNA (Wilson and Blitchington…”
Reductive dechlorination of β‐hexachlorocyclohexane (β‐HCH) by a Dehalobacter species in coculture with a Sedimentibacter sp. (2005, neutral): “[28].”
Screening of isolated potential probiotic lactic acid bacteria for cholesterol lowering property and bile salt hydrolase activity (2016, methodology): “Molecular methods are important for bacterial identification (Drancourt et al., 2000; Sghir et al., 2000; Greetham et al., 2002; Heilig et al., 2002) and possibly more accurate for LAB than the conventional phenotypic methods.”