Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system

By Kabin Xie, Bastian Minkenberg, Yinong Yang. Proceedings of the National Academy of Sciences of the United States of America, 2015

1,339 citations recorded by the source corpus.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/082c37622fed9708ed1dd4db3bca2572ba6f5a18

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In Molecular Biology

Citations in the corpus

Multiplex gene editing by CRISPR-Cpf1 through autonomous processing of a single crRNA array (2016, background): “, 2014), incorporation of long recognition sites for endogenous nucleases (Xie et al., 2015) or coexpression of a specialized nuclease cutting a small sequence between sgRNAs (Nissim et al.”
CRISPR/Cas Genome Editing and Precision Plant Breeding in Agriculture. (2019, methodology): “This has been achieved using a polycistronic gene construct in which the gRNA is interspersed with ribozyme sites (35), Csy4 recognition sites (14), or transfer RNA sequences (173), which are processed in the plant cell to release mature gRNAs for editing.”
A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants. (2015, methodology): “During our preparation of this article, a strategy for generating multiple target-sgRNAs from linked polycistronic tRNA–gRNA units (each 173 bp) using the endogenous tRNA processing system was reported (Xie et al., 2015).”
Genome Editing—Principles and Applications for Functional Genomics Research and Crop Improvement (2017, methodology): “In contrast, CRISPR/Cas9 can efficiently target multiple sites by providing a Cas9 cDNA and multiple sgRNAs in a single expression cassette (Wang et al., 2013; Xie et al., 2015).”
The Sequence of 1504 Mutants in the Model Rice Variety Kitaake Facilitates Rapid Functional Genomic Studies (2017, background): “, 2012), CRISPR/Cas9 genome editing (Jiang et al., 2013; Miao et al., 2013; Xie et al., 2015), chemical induction, such as ethyl methanesulfonate (EMS) (Henry et al.”
A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation1[OPEN] (2015, background): “Zinc finger nickases and transcription activator-like effector (TALE) nickases have been made by generating a D450A mutation in the FokI nuclease domain, but these nickases appear to have noticeable residual nuclease activity (Ramirez et al., 2012; Wang et al., 2012; Wu et al., 2015).”
Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation (2015, methodology): “Since methods have been developed to assemble multiple sgRNAs [26, 37, 54, 56, 57], highly efficient expression of multiple sgRNAs in a cell has not been a problem.”