Multiplex gene editing by CRISPR-Cpf1 through autonomous processing of a single crRNA array
By Bernd Zetsche, Matthias Heidenreich, Prarthana Mohanraju, I. Fedorova, Jeroen Kneppers, Ellen M. DeGennaro, Nerges Winblad, Sourav R. Choudhury, Omar Abudayyeh, Jonathan Gootenberg and 5 more. Nature Biotechnology, 2016
686 citations recorded by the source corpus.
Targeting of multiple genomic loci with Cas9 is limited by the need for multiple or large expression constructs. Here we show that the ability of Cpf1 to process its own CRISPR RNA (crRNA) can be used to simplify multiplexed genome editing. Using a single customized CRISPR array, we edit up to four genes in mammalian cells and three in the mouse brain, simultaneously.
Subfields: Molecular Biology
View Paper: https://www.semanticscholar.org/paper/91cbc1c1735256ce2abcaad780b24e8ac19ccc40
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Citations in the corpus
- Perturb-Seq: Dissecting Molecular Circuits with Scalable Single-Cell RNA Profiling of Pooled Genetic Screens. (2016, background): “Future work could leverage the ability of Cpf1 to autonomously process an entire array and deliver several sgRNAs (or an unprocessed array) on one construct (Zetsche et al., 2016).”
- CRISPR-Cas12a: Functional overview and applications (2020, background): “[52] Zetsche B,HeidenreichM,Mohanraju P, Fedorova I, Kneppers J, DeGennaro EM, et al.”
- Non-viral delivery systems for CRISPR/Cas9-based genome editing: challenges and opportunities (2018, background): “Recently, an alternative CRISPR-based nuclease Cpf1 has been discovered and share many similar advantages and shortcomings of Cas9 [29, 30].”