Microbial community diversity associated with the intestinal mucosa of farmed rainbow trout (Oncoryhnchus mykiss Walbaum)
By D. Merrifield, Dean Burnard, G. Bradley, S. Davies, R. Baker. 2009
120 citations recorded by the source corpus.
Bacterial communities from the intestinal tract of rainbow trout were investigated to assess transient and resident microbial communities using both culture-based and culture-independent techniques. Viable counts attached to the intestinal mucosa were in the range of log 4.77–5.38 and log 6.67–6.79 CFU g−1 in the intestinal contents. Pseudomonas spp. and Enterobacteriaceae constituted nearly 80% of the allochthonous population but <60% of the autochthonous populations. This coincided with an elevated mucosal level of a group of Gram-positive rods from ∼2% in the digesta to 25–35% on the mucosa. This group was identified by 16S rRNA as Arthrobacter aurescens and Janibacter spp. HTCC2649. Analysis of denaturing gradient gel electrophoresis banding patterns showed complex communities in all intestinal regions. Similarity coefficients showed that mucosal communities were ∼70% similar to digesta communities and yet due to the presence of bands found uniquely either in the digesta or on the mucosa, the communities are distinctly different. Scanning electron microscopy confirmed mucosal bacterial populations and highlighted a possible localized colonization between mucosal folds. The study highlights the complexity of resident microbial communities that have not been fully explored in previous rainbow trout studies; this is especially true with probiotic/prebiotic investigations.
Subfields: Aquatic Science, Cancer Research, Immunology
View Paper: https://www.semanticscholar.org/paper/05c6655e3681e588973b931762cd489278be9994
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Citations in the corpus
- Effect of dietary components on the gut microbiota of aquatic animals. A never‐ending story? (2016, methodology): “Electron microscopy Attention has been paid to the importance of scanning electron microscopy (SEM) investigations in gut studies of the microbiota (Ringø & Olsen 1999; Ringø et al. 2001a,b, 2002, 2003, 2007a,b; ; Merrifield et al. 2009a,b, 2011a,b; Harper et al. 2011).”
- Evidence for a core gut microbiota in the zebrafish (2011, methodology): “The nature of our study required that we limited our UniFrac analysis to those fish species for which complete (that is, nondereplicated) 16S rRNA clone libraries were available (Table 1), however, these same bacterial classes have been also observed in the intestinal microbiotas of other teleost fi”