Optical Sectioning Deep Inside Live Embryos by Selective Plane Illumination Microscopy

By Jan Huisken, J. Swoger, F. Del Bene, Joachim Wittbrodt, E. Stelzer. Science, 2004

2,304 citations recorded by the source corpus.

Subfields: Biomedical Engineering, Biophysics

View Paper: https://www.semanticscholar.org/paper/054281b0ddc4e3931d8c45ffc54d3596e6ad0ada

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Citations in the corpus

Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain (2007, background): “Other approaches did not yield cellular resolution in the past or are only applicable to small transparent organism”
Labkit: Labeling and Segmentation Toolkit for Big Image Data (2021, methodology): “In recent years, new and powerful microscopy and sample preparation techniques have emerged, such as light-sheet (Huisken et al., 2004), super-resolution microscopy (Hell and Wichmann, 1994; Gustafsson, 2000; Betzig et al.”
Fluorescence molecular imaging. (2006, background): “In vivo near-infrared fluorescence imaging of integrin a,alpha(v)beta(3) in brain tumor xenografts.”
Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain (2011, positive): “In particular, we suggest the utility of Sca l e-treated samples for 'light sheet' ultramicroscopy, a technique that can gather three-dimensional image data quickl”
A guide to super-resolution fluorescence microscopy (2010, methodology): “Thus, the embryogenesis of flies and zebrafish was successfully imaged with an isotropic resolution using selective plane illumination microscopy (SPIM) and an orthogonal camera-based detection (Huisken et al., 2004; Keller et al., 2008).”
Microscopy with self-reconstructing beams (2010, methodology): “Furthermore, a light-sheet-based type of microscope is faster in terms of image acquisition than a point-scanning confocal microscope, because it can be driven in a plane-scanning configuration by using a cylindrical len”
Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination (2011, methodology): “Although this has been successful for imaging large multicellular organisms at single-cell resolutio”
The light-sheet microscopy revolution (2018, methodology): “The introduction of the light sheet microscope, as it is now understood, was described in 2004 [1], which started the rapid growth of the technique.”
Going deeper than microscopy: the optical imaging frontier in biology (2010, background): “Also pursuing the goal of easy-to-implement microscopic imaging of large samples, Huisken et al”