Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain

By H. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. Deussing, M. Eder, W. Zieglgänsberger, K. Becker. Nature Methods, 2007

1,218 citations recorded by the source corpus.

Subfields: Biophysics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/50d8443905ec20675788f3043079268aa18236d2

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Citations in the corpus

Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain (2011, methodology): “BABB (a mixture of benzyl-alcohol and benzyl-benzoate) is another clearing solution that has been used with ultramicroscop”
Single-Cell Phenotyping within Transparent Intact Tissue Through Whole-Body Clearing (2014, neutral): “Please cite this article in press as: Yang et al., Single-Cell Phenotyping within Transparent Intact Tissue through Whole-Body Clearing, Cell (2014), http://dx.doi.org/10.1016/j.cell.2014.07.017 methods and optimized protocols were only presented for clearing brain tissue, and occasionally for the s”
Going deeper than microscopy: the optical imaging frontier in biology (2010, methodology): “Similarly to OPT, SPIM has also been combined with methods for tissue clearanc”
Roadmap on structured light (2016, background): “One way ofsolvingthis problem is to apply clearing agents [98], which seems incompatible with life”
High-throughput dual-colour precision imaging for brain-wide connectome with cytoarchitectonic landmarks at the cellular level (2016, positive): “Thus far, CUBIC is employed in light-sheet microscopy 19 to image counterstained brains and has not shown potential for use in detailed whole-brain labelling and counterstaining images at axonal resolution.”
Labkit: Labeling and Segmentation Toolkit for Big Image Data (2021, background): “…microscopy (Hell and Wichmann, 1994; Gustafsson, 2000; Betzig et al., 2006; Hess et al., 2006; Rust et al., 2006), modern tissue clearing (Dodt et al., 2007; Hama et al., 2011), or serial section scanning electron microscopy (Denk and Horstmann, 2004; Knott et al., 2008) enabling…”
Clarifying Tissue Clearing (2015, neutral): “To date, methylsalicilate, benzyl alcohol, benzyl benzoate, dichloromethane, and dibenzyl ether have been used as final clearing solutions (Becker et al., 2012; Dodt et al., 2007; Ertürk et al., 2012a, 2012b; Renier et al., 2014; Spalteholz, 1914; Steinke and Wolff, 2001).”
Advanced CLARITY for rapid and high-resolution imaging of intact tissues (2014, methodology): “As a step in this direction, new methods have emerged to increase tissue transparency (Dodt et al., 2007; Hama et al., 2011; Ke et al., 2013) by chemically reducing the scattering of light traveling through the tissue sample.”