Directed evolution of antibody fragments with monovalent femtomolar antigen-binding affinity.

By E. Boder, K. Midelfort, K. Wittrup. Proceedings of the National Academy of Sciences of the United States of America, 2000

689 citations recorded by the source corpus.

Subfields: Immunology, Molecular Biology, Radiology, Nuclear Medicine and Imaging

View Paper: https://www.semanticscholar.org/paper/0497861f7a0f00e03a3bfda127f20d72c95291f4

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In Immunology

In Molecular Biology

In Radiology, Nuclear Medicine and Imaging

Citations in the corpus

Computational design of antibody-affinity improvement beyond in vivo maturation (2007, background): “3, with over 1,000-fold affinity improvement and 14 mutation”
Beyond natural antibodies: the power of in vitro display technologies (2011, background): “has become relatively routine, with one study describing an antibody in the femtomolar rang”
Cognizance of Molecular Methods for the Generation of Mutagenic Phage Display Antibody Libraries for Affinity Maturation (2019, methodology): “[94] Single-site saturation mutagenesis was performed using PFunkel mutagenesis for TNF, pertussis toxin, and TROP2 mutant libraries ND ND ND Selection against 32 nM scFv (TNF), 5 nM anti-pertussis, 22 nM Fab (TROP2) using FACS [216] Affibody (Aβ) mutant libraries construction using SlonoMax® ND 107”
Selecting and screening recombinant antibody libraries (2005, methodology): “In combination with random mutagenesis methods to diversify the V H and V L genes (see below), the yeast-display method has yielded the highest affinity (48 fM) for any antibod”
Repertoire Analysis of Antibody CDR-H3 Loops Suggests Affinity Maturation Does Not Typically Result in Rigidification (2017, background): “3 (66), but the crystal structures of 4M5.”
Isolating and engineering human antibodies using yeast surface display (2006, background): “In addition, an antibody to fluorescein has been engineered to femtomolar affinity, the highest affinity reported so fa”
OptCDR: a general computational method for the design of antibody complementarity determining regions for targeted epitope binding. (2010, methodology): “…bind the peptide from the capsid of hepatitis C. antibodies have been used as the test system by several experimental directed evolution efforts (Boder et al., 2000; Jermutus et al., 2001; Fukuda et al., 2006) with different display technologies to improve the binding of an scFv derived from…”