Isolating and engineering human antibodies using yeast surface display

By Ginger Chao, W. L. Lau, B. Hackel, Stephen Sazinsky, S. Lippow, D. Wittrup. 2006

913 citations recorded by the source corpus.

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Citations in the corpus

Computational design of a protein-based enzyme inhibitor. (2013, unknown): “…on different scaffolds were selected (Table S1), and yeast codon-optimized genes encoding the designs were synthesized and expressed in the EBY100 yeast strain as a fusion between N-terminal Aga2p for surface display and a C-terminal Myc epitope tag for detection with a fluorescent antibody [21].”
Nonspecificity in a nonimmune human scFv repertoire (2017, methodology): “using described previously yeast display techniques.(37,38) Initial rounds of sorting were conducted using Dynabeads biotin binder beads (Life Technologies), ensuring at least ten-fold coverage of the library or population size in each round.”
An improved yeast transformation method for the generation of very large human antibody libraries. (2010, background): “…yeast, mammalian cell and ribosomal displays; Becker and Guarente, 1991; Griffiths et al., 1994; Gietz et al., 1995; Feldhaus et al., 2003; Chao et al., 2006; Gai and Wittrup, 2007) offers a valuable parallel approach and may present the best alternative in cases where the target antigen…”
Principles for computational design of binding antibodies (2017, methodology): “To increase the binders ’ affinity for their designated targets, we used error-prone PCR to introduce one to four random mutations in the scFv-coding genes of the three designs, followed by yeast display and fluorescence-activated cell sorting of higher affinity variants (19).”
Protein model discrimination using mutational sensitivity derived from deep sequencing. (2012, background): “Even in the absence of any binding partner, cell-surface expression of an epitope-tagged protein can be used as a convenient readout (Chao et al., 2006).”
Neoantigens: promising targets for cancer therapy (2023, methodology): “Using a competitive selection technique, clones specific for mutant peptides bound to predetermined HLA types were subsequently identified.(441,442) A high-throughput genetic platform, PresentER, is comprised of minigenes that encode MHC-I peptide libraries.”
Sampling and energy evaluation challenges in ligand binding protein design (2017, unknown): “01% BSA in 100 mL PBSF buffer.(11) In a 1.”
Computational Design of Ligand Binding Proteins with High Affinity and Selectivity (2013, methodology): “Surface protein expression was induced 22 and cells were labelled with anti-c-Myc-FITC and sorted for protein expression.”
Surrogate Wnt agonists that phenocopy canonical Wnt/β-catenin signaling (2017, unknown): “To induce the display of B12 on the yeast surface, cells were growing in SGCAA medium 45,46 for 2 days at 20 °C. 1 × 10 6 yeast cells per condition were washed with PBE (PBS, 0.5% BSA, 2 mM EDTA), and stained separately with 0.06–1,000 nM biotinylated FZD1/4/5/7/8/10 CRDs for 2 h at 4 °C.”
Efficient proximity labeling in living cells and organisms with TurboID (2018, unknown): “For yeast-display (Figure 1c, e and Supplementary Figure 3, 4a), S. cerevisiae strain EBY100 was cultured according to previously published protocols 40 .”