Selecting and screening recombinant antibody libraries

By H. Hoogenboom. Nature Biotechnology, 2005

983 citations recorded by the source corpus.

Subfields: Molecular Biology, Radiology, Nuclear Medicine and Imaging

View Paper: https://www.semanticscholar.org/paper/00630fbb3ecedfb8d8982cea5f428dee73f35698

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

In Radiology, Nuclear Medicine and Imaging

Citations in the corpus

Mining human antibody repertoires (2010, background): “Over the past 20 years, phage display has proven to be the most robust and versatile mining tool for human antibody repertoires, yielding human mAbs to virtually any antigen of interest.(8) The process of multiple rounds of selection on a purified antigen or on antigen-expressing cells, referred to ”
An improved yeast transformation method for the generation of very large human antibody libraries. (2010, background): “Although yeast library sizes in the 109 range have been previously reported (Hoogenboom, 2002, 2005; He and Khan, 2005), typical yeast display library sizes ( 107) tend to be significantly smaller than those achieved by other display technologies (Griffiths et al., 1994; Gietz et al., 1995).”
Potent antibody therapeutics by design (2006, background): “and because fragments are commonly used in the engineeri ng of antibody propertie”
OptMAVEn – A New Framework for the de novo Design of Antibody Variable Region Models Targeting Specific Antigen Epitopes (2014, background): “More recently, complete human antibodies have been designed using directed evolution techniques [6,7] that mimic the natural selection of the process to evolve antibodies towards a desired property.”
Antibody-Based Sensors: Principles, Problems and Potential for Detection of Pathogens and Associated Toxins (2009, background): “Recombinant antibodies, generated through the use of phage display technology and the biopanning of antibody repertoires (libraries) against a target of interest [33, 34] , have been selected for the detection of a range of structurally diverse antigens, including proteins [35] , haptens [36] and ca”
The CamSol method of rational design of protein mutants with enhanced solubility. (2015, methodology): “Standard approaches to achieve this objective are based on the optimization of the solubility by phage display and heat denaturation [7,12,15,29,30].”
Generation and analyses of human synthetic antibody libraries and their application for protein microarrays. (2016, background): “As the success of generating a binder is directly related to library size (Hoogenboom, 2005), the trend has been to create larger and larger libraries.”
Addressing polyspecificity of antibodies selected from an in vitro yeast presentation system: a FACS-based, high-throughput selection and analytical tool. (2013, background): “While the generation of target-binding antibodies has become more routine with improvements of in vivo and in vitro discovery technologies (Hoogenboom, 2005; Carter, 2006), there are additional attributes that often preclude antibody leads from becoming successful drugs (Jacobs et al., 2010; Wu et…”
Isolating and engineering human antibodies using yeast surface display (2006, background): “Various technologies for screening recombinant antibody libraries and their relative strengths and weaknesses have been reviewe”