Beyond natural antibodies: the power of in vitro display technologies

By A. Bradbury, S. Sidhu, S. Dübel, J. McCafferty. 2011

536 citations recorded by the source corpus.

In vitro display technologies, best exemplified by phage and yeast display, were first described for the selection of antibodies some 20 years ago. Since then, many antibodies have been selected and improved upon using these methods. Although it is not widely recognized, many of the antibodies derived using in vitro display methods have properties that would be extremely difficult, if not impossible, to obtain by immunizing animals. The first antibodies derived using in vitro display methods are now in the clinic, with many more waiting in the wings. Unlike immunization, in vitro display permits the use of defined selection conditions and provides immediate availability of the sequence encoding the antibody. The amenability of in vitro display to high-throughput applications broadens the prospects for their wider use in basic and applied research.

Subfields: Biotechnology, Molecular Biology, Radiology, Nuclear Medicine and Imaging

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

In Radiology, Nuclear Medicine and Imaging

Citations in the corpus

Protein design and variant prediction using autoregressive generative models (2021, background): “In order to circumvent these limitations, there has been an emphasis on very large libraries (~10 9 – 10 10 ) to achieve these desired features 19,20 .”
Cognizance of Molecular Methods for the Generation of Mutagenic Phage Display Antibody Libraries for Affinity Maturation (2019, background): “Despite the fact that combinatorial libraries are able to produce antibodies with improved affinities and specificities, it is not always possible to gain antibodies with those desirable features [120].”
Utilizing graph machine learning within drug discovery and development (2021, methodology): “In phage display, selection works by physical capture and elution [195]; for cell-based display technologies (like yeast display), fluorescence-activated cell sorting (FACS) is utilised for selection [196].”
Naïve Human Antibody Libraries for Infectious Diseases (2018, background): “The application of in vitro display technologies like phage display has allowed the generation of fully human mAbs in greater amounts and in a more controlled manner [53, [59] [60] [61] .”
The CamSol method of rational design of protein mutants with enhanced solubility. (2015, background): “Antibodies, in particular, can be obtained with well-established methods, including immunization or phage and associated display methods, against virtually any target of therapeutic interest, which they bind with high affinity and specificity [5–9].”