Analysis of gene function in somatic mammalian cells using small interfering RNAs.

By Sayda M. Elbashir, J. Harborth, K. Weber, Thomas Tuschl. Methods, 2002

1,188 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/01ff1629b54befdb000b1d365ddb0752dbb77cfb

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In Molecular Biology

Citations in the corpus

3′ UTR seed matches, but not overall identity, are associated with RNAi off-targets (2006, background): “Because early studies in RNAi established that single-base-pair mismatches could dramatically alter siRNA functionalit”
Rational siRNA design for RNA interference (2004, background): “Previous work had correlated low G/C content within target mRNA regions with efficient siRNA silencin”
Differentiating the functional role of hypoxia‐inducible factor (HIF)‐1α and HIF‐2α (EPAS‐1) by the use of RNA interference: erythropoietin is a HIF‐2α target gene in Hep3B and Kelly cells (2004, methodology): “As a negative control, we used a siRNA targeting the firefly luciferase coding sequence (cds) of the pGL2 vectors (25) (sense siRNA 5′ CGUACGCGGAAUACUUCGAdTdT; synthesis by Eurogentec, Bruxelles, Belgium).”
Gene silencing in mammals by small interfering RNAs (2002, background): “cgi?Q=lmn*%20not%203930%20not%204002%20not%204003&ORG=Hs">lamin can be accomplished in HeLa cell”
Cancer siRNA therapy by tumor selective delivery with ligand-targeted sterically stabilized nanoparticle. (2004, methodology): “(2), validated to lack significant interfering homology using BLAST analysis, and synthesized and purified by Dharmacon (Lafayette, CO).”
On the art of identifying effective and specific siRNAs (2006, background): “Shortly after the discovery that siRNA duplexes can specifically silence mammalian genes, it was thought that almost any target-complementary siRNA effectively and specifically silences its cognate target gen”
Thermodynamics of RNA-RNA Binding (2006, background): “Several groups have proposed basically empirical rules for designing functional siRNAs, see e.g. Elbashir et al. (2002) and Reynolds et al. (2004), but the efficiency of siRNAs generated using these rules is highly variable.”
ZBP2 Facilitates Binding of ZBP1 to β-Actin mRNA during Transcription (2007, background): “RNAi is a convenient and powerful way to study loss of protein function in cultured cells (15, 16).”
Single-stranded antisense siRNAs guide target RNA cleavage in RNAi. (2002, background): “, and development (Zou et al., 1998; Sharma et al., 2001; Deng and Lin, 2002; in D. melanogaster (Caplen et al., 2001; Elbashir et al., 2002).”
EB1 and APC bind to mDia to stabilize microtubules downstream of Rho and promote cell migration (2004, methodology): “Cells were transfected with 200 nM EB1 siRNA (nucleotides 181–199; 5′-AAGUGAAAUUCCAAGCUAAGC-3′ (synthesized by Dharmacon, Lafayette, CO)) using 14 μl Transit TKO (Mirus, Madison, WI) as describe”
Functional siRNAs and miRNAs exhibit strand bias. (2003, neutral): “5 kcal/mol lower when energy penalties for the presence AA(N19) rule (Elbashir et al., 2002) and may have some bias in the target site selection.”