Impaired Pressure Sensation in Mice Lacking TRPV4*

By Makoto Suzuki, Atsuko Mizuno, K. Kodaira, M. Imai. Journal of Biological Chemistry, 2003

756 citations recorded by the source corpus.

The sensation of pressure, mechanosensation, in vertebrates remains poorly understood on the molecular level. The ion channel TRPV4 is in the TRP family and is a candidate for a mechanosensitive calcium-permeable channel. It is located in dorsal root ganglia. In the present study, we show that disrupting the Trpv4 gene in mice markedly reduced the sensitivity of the tail to pressure and acidic nociception. The threshold to noxious stimuli and the conduction velocity of myelinated nerve responding to stimuli were also impaired. Activation of unmyelinated nerve was undetected. However, the mouse still retained olfaction, taste sensation, and heat avoidance. The TRPV4 channel expressed in vitro in Chinese hamster ovary cells was opened by low pH, citrate, and inflation but not by heat or capsaicin. These data identify the TRPV4 channel as essential for the normal detection of pressure and as a receptor of the high-threshold mechanosensory complex.

Subfields: Cellular and Molecular Neuroscience, Nutrition and Dietetics, Sensory Systems

View Paper: https://www.semanticscholar.org/paper/fffda298d20f95e0badbe80ff18ed08540b1c9f7

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Cellular and Molecular Neuroscience

1. Ardem Patapoutian
2. A. Dhaka
3. M. Caterina
4. Ellen A. Lumpkin
5. Veena Viswanath
6. D. Julius

In Nutrition and Dietetics

1. Ardem Patapoutian
2. M. Caterina
3. Veena Viswanath

In Sensory Systems

1. Ardem Patapoutian
2. A. Dhaka
3. Veena Viswanath
4. M. Caterina
5. Ellen A. Lumpkin
6. D. Julius
7. Clifford J. Woolf

Citations in the corpus

• Trp ion channels and temperature sensation. (2006, background): “…tissues including DRG neurons, skin, and the hypothalamus, although expression in the DRG is controversial; some groups have reported expression and others could not detect it (Alessandri-Haber et al. 2003, Delany et al. 2001, Guler et al. 2002, Liedtke et al. 2000, Suzuki et al. 2003).”
• Mechanisms of sensory transduction in the skin (2007, background): “is consistent with a role in cutaneous touch, disrupting Trpv4 expression in mice has only modest effects on acute mechanosensory threshold”
• An introduction to TRP channels. (2006, neutral): “PI(4,5)P2 TRPM7 20μM agonist (104) I HEK,CHO PI(4,5)P2 TRPM5 10μM 1.”