Quantification of Glycosidase Activity in Selected Strains of Brettanomyces bruxellensis and Oenococcus oeni

By A. Mansfield, B. Zoecklein, R. S. Whiton. American Journal of Enology and Viticulture, 2002

89 citations recorded by the source corpus.

Brettanomyces bruxellensis and lactic acid bacteria are common microorganisms capable of modifying wine aroma and flavor. The activity of β-glucosidase against p -nitrophenyl-β-D-glucopyranoside was determined in a model system for 14 strains of Brettanomyces bruxellensis yeast and 9 strains of lactic acid bacteria (Oenococcus oeni) . All Brettanomyces strains and 7 Oenococcus strains exhibited enzymatic activity against this substrate. B. bruxellensis β-glucosidase activity was primarily intracellular; O. oeni showed some extracellular activity. Strains showing activity greater than 1000 nmole mL -1 g dry cell mass -1 24 hr -1 for Brettanomyces , or 100 nmole mL -1 g dry cell mass -1 24 hr -1 for Oenococcus , were evaluated for their effect on native Viognier grape glycosides. Neither genus was active on Viognier grape glycosides.

Subfields: Biochemistry, Food Science, Pharmacology

View Paper: https://www.semanticscholar.org/paper/fd6d0e635910df7e84255ef6dd08f4a3a0fcb436

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In Food Science

1. M. Toit
2. I. S. Pretorius
3. E. Bartowsky
4. P. Henschke
5. A. Oelofse
6. L. Engelbrecht
7. E. Lerm
8. N. Jolly

In Pharmacology

1. I. S. Pretorius

Citations in the corpus

• Significance of Brettanomyces and Dekkera during winemaking: a synoptic review. (2016, background): “A few reports are available that show glycosidic activity (b-glucosidase) amongst Brettanomyces/ Dekkera strains (Fugelsang et al., 1993; McMahon et al., 1999; Mansfield et al., 2002; Potgieter, 2004; Fia et al., 2005).”
• Yeast and bacterial modulation of wine aroma and flavour (2005, background): “No release could be demonstrated, for example, in a Viognier wine (Mansfield et al. 2002), but release could be shown in a highly aromatic Muscat variety (Ugliano et al. 2003).”
• Malolactic Fermentation: The ABC’s of MLF (2016, background): “McMahon et al. (1999) found no glycosidase activity in commercial O. oeni cultures, whereas Mansfield et al. (2002) saw β-glucosidase activity in a model system, but none of the strains were active on Viognier grape glycosides.”