Telomerase expression extends the proliferative life-span and maintains the osteogenic potential of human bone marrow stromal cells

By J. L. Simonsen, C. Rosada, N. Serakıncı, J. Justesen, K. Stenderup, S. Rattan, T. Jensen, M. Kassem. Nature Biotechnology, 2002

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Aging of mesenchymal stem cells. (2006, background): “In contrast, forced telomerase expression leads to an extended life span and enhanced differentiation potential in human MSC (Shi et al., 2002; Simonsen et al., 2002).”
Long-term cultures of bone marrow-derived human mesenchymal stem cells frequently undergo spontaneous malignant transformation. (2009, background): “According to several reports, the telomerase hTERT gene alone does not induce malignant transformation (33, 35).”
Molecular and cellular characterisation of highly purified stromal stem cells derived from human bone marrow (2003, background): “Recent studies have shown that human BMSSCs lose telomerase activity during ex vivo expansion, whereas enforced telomerase activity greatly enhanced the proliferative life-span and osteogenic potential of cultured BMSSCs (Shi et al., 2002; Simonsen et al., 2002).”
Adult mesenchymal stem cells: characterization, differentiation, and application in cell and gene therapy (2004, background): “Several studies have shown that forced ectopic expression of human telomerase reverse transcriptase (hTERT) in postnatal MSCs could extend their life span to more than 260 population doublings, while maintaining their osteogenic, chondrogenic, adipogenic, neurogenic, and stromal differentiation pote”
Adult mesenchymal stem cells and cell-based tissue engineering (2002, background): “but also to increase their osteogenic differentiation potential [44,45].”