Derivation of embryonic germ cells and male gametes from embryonic stem cells

By N. Geijsen, Melissa Horoschak, Kitai Kim, J. Gribnau, K. Eggan, G. Daley. Nature, 2004

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Embryonic stem cell differentiation: emergence of a new era in biology and medicine. (2005, methodology): “In the second study, germ cells generated in serum-stimulated EBs were isolated on the basis of SSEA1 expression and cultured in the presence of retinoic acid, LIF, bFGF, and c-kit ligand (Geijsen et al. 2004).”
Reciprocal Transcriptional Regulation of Pou5f1 and Sox2 via the Oct4/Sox2 Complex in Embryonic Stem Cells (2005, background): “In addition, ESCs can also differentiate into a variety of different cell types when cultured in vitro (17, 19, 20, 40, 52).”
Human embryonic stem cells with biological and epigenetic characteristics similar to those of mouse ESCs (2010, result): “This is in contrast to mESCs, which are inefficient in activating these markers and require prior EB formation (7, 17, 27).”
Oct4-induced pluripotency in adult neural stem cells. (2009, neutral): “Previous reports have demonstrated the in vitro generation of GCs from murine ESCs (Geijsen et al., 2004; Hübner et al., 2003).”
Embryonic stem cells: prospects for developmental biology and cell therapy. (2005, background): “Several seminal discoveries during the past 25 years can be regarded not only as major breakthroughs for cell and developmental biology, but also as pivotal events that have substantially influenced our view of life: 1) the establishment of embryonic stem (ES) cell lines derived from mouse (108, 221”