A Gateway Cloning Vector Set for High-Throughput Functional Analysis of Genes in Planta[w]

By M. Curtis, Ueli Grossniklaus. Plant Physiology, 2003

2,688 citations recorded by the source corpus.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/f640afd3488eb756451592b93dc5b060852511fd

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Citations in the corpus

Specificity of ARGONAUTE7-miR390 interaction and dual functionality in TAS3 trans-acting siRNA formation. (2008, methodology): “The resulting products were cloned in pENTR (Invitrogen), followed by recombination into the plant transformation vector pMDC32, pMDC163 (a GUS fusion vector), or pMDC99 (Curtis and Grossniklaus, 2003).”
Repression of AUXIN RESPONSE FACTOR10 by microRNA160 is critical for seed germination and post-germination stages. (2007, background): “ABA suppresses expression of the auxin-responsive reporter gene DR5:GUS in lateral roots (De Smet et al., 2003).”
A CRISPR/Cas9 toolkit for multiplex genome editing in plants (2014, background): “The vectors pCAMBIA1300/ 2300/3300 and their derivatives (including the Gatewaycompatible pMDC series) are some of the most widely used binary vectors for a variety of plant species [42,43], and some plant transformation protocols have been specifically optimized based on these vectors.”
Regulation of Phosphate Homeostasis by MicroRNA in Arabidopsis[W] (2005, methodology): “The miR399 precursors were subcloned into pMDC32 (Curtis and Grossniklaus, 2003) harboring a dual cauliflower mosaic virus 35S promoter, a strong constitutive promoter, with the use of Gateway recombination technology (Invitrogen).”
PHYTOCHROME-INTERACTING FACTOR 4 (PIF4) regulates auxin biosynthesis at high temperature (2011, methodology): “Resulting plasmids were sequenced and then recombined with pMDC43 (36) using Gateway LR Clonase to produce vectors containing the 2× 35S promoter driving expression of N-terminal GFP-SAUR fusion proteins.”
AtHMA3, a P1B-ATPase Allowing Cd/Zn/Co/Pb Vacuolar Storage in Arabidopsis1[W] (2008, methodology): “Gateway ends were added by PCR, and the construction was then subcloned in the plant Gateway vector pMDC43 (Curtis and Grossniklaus, 2003) by LR Clonase recombination (Invitrogen).”
A Downstream Mediator in the Growth Repression Limb of the Jasmonate Pathway[W][OA] (2007, methodology): “For overexpression, the resulting cDNAs were cloned into the vector pMDC32 ( Curtis and Grossniklaus, 2003 ), which was”
A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation1[OPEN] (2015, background): “Because such T-DNA destination vectors have been previously developed by others (Curtis and Grossniklaus, 2003; Earley et al., 2006), our work focuses on making entry clones for the first two modules (Fig.”