Conventional PCR and Real-time Quantitative PCR Detection of Helminthosporium Solani in Soil and on Potato Tubers

By D. Cullen, Alison K. Lees, Ian K. Toth, J. Duncan. European journal of plant pathology, 2001

140 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/f6152ec5ae9a12942aabf5232df662fb0b0ab53d

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Cell Biology

In Plant Science

Citations in the corpus

Widespread Phytophthora infestations in European nurseries put forest, semi-natural and horticultural ecosystems at high risk of Phytophthora diseases (2016, methodology): “…the technologies and databases became available, confirmation of identifications was made using polymerase chain reaction (PCR) to amplify ITS-rDNA, followed by sequencing and comparison against sequences in databases such as GenBank and PhytophthoraDB (e.g. Cooke et al. 2000; Cullen et al. 2001).”
Development and application of a PCR‐based ‘molecular tool box’ for the identification of Phytophthora species damaging forests and natural ecosystems (2007, methodology): “DNA extraction from pure cultures, leaves, soil and water DNA was extracted from pure cultures of Phytophthora and Pythium (Schena & Cooke, 2006), naturally and artificially infected leaves (Schena et al., 2006) and from naturally infested soil samples (Cullen et al., 2001).”
Real-time quantitative PCR: a new technology to detect and study phytopathogenic and antagonistic fungi (2004, methodology): “solani (Cullen et al., 2001), Colletotrichum coccodes (Cullen et al.”
Identification and Detection of Rosellinia Necatrix by Conventional and Real-time Scorpion-PCR (2002, unknown): “For each sample, DNA extraction was performed in triplicate by using the protocol described by Cullen et al. (2001) with minor modifications.”
Detection of Phytophthora nicotianae and P. citrophthora in Citrus Roots and Soils by Nested PCR (2002, methodology): “For each sample, DNA extraction was performed in triplicate by using the protocol described by Cullen et al. (2001) with minor modifications.”
In‐field distribution of Plasmodiophora brassicae measured using quantitative real‐time PCR (2012, background): “The intersample reproducibility (Table 5) for the assay developed in this study was similar to, or better than, that of assays for other plant pathogens quantified in soil samples (Cullen et al., 2001, 2002; Atkins et al., 2003, 2005; Zang et al., 2006).”
Development of conventional and quantitative real‐time PCR assays for the detection and identification of Rhizoctonia solani AG‐3 in potato and soil (2002, background): “, 1999) and Helminthosporium solani (Cullen et al., 2001), and Colletotrichum coccodes (Cullen et al.”
Molecular and Morphological Diversity of Fusarium Species in Finland and North-Western Russia (2004, unknown): “The ultimate goal of our future studies is to develop routine DNA extraction and quantitative PCR methods (Cullen et al., 2001; Dan et al., 2001, Schnerr et al., 2001) to enable molecular detection and identification of pathogenic and toxigenic Fusarium species or isolates (Bakan et al., 2002;…”
Detection of Colletotrichum coccodes from soil and potato tubers by conventional and quantitative real-time PCR (2002, methodology): “High molecular-weight (23 kb) DNA of a suitable purity for PCR was recovered from soil (four different soil types) and potato tubers within 3 h by the modified extraction method (Cullen et al., 2001).”
Real-time detection of Phytophthora nicotianae and P. citrophthorain citrus roots and soil (2004, background): “…techniques, Taq-Man (Lee et al.,1993), Molecular beacons (Tyagi and Kramer, 1996), and Scorpion-PCR (Whitcombe et al., 1999) are the most promising with increasing reports regarding their application in plant pathology (Bates et al.,2001; Cullen et al.,2001; Schena et al., 2002a, in press).”