Identification and Detection of Rosellinia Necatrix by Conventional and Real-time Scorpion-PCR

By L. Schena, Franco Nigro, A. Ippolito. European journal of plant pathology, 2002

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Real-time detection of Phytophthora nicotianae and P. citrophthorain citrus roots and soil (2004, methodology): “…and it has been utilised both in human pathology to detect HIV virus (Saha et al.,2001) and in plant pathology to identify and detect antagonistic (Schena et al., 2002a) or pathogenic fungi (Ippolito et al.,2000; Bates and Taylor, 2001; Nigro et al., 2002) and viruses (Finetti Sialer et al.,…”
Real-time quantitative PCR: a new technology to detect and study phytopathogenic and antagonistic fungi (2004, methodology): “These chemistries can be grouped into amplicon sequence non-specific (SYBR Green I) and sequence specific (TaqMan, Molecular beacons, and Scorpion-PCR) methods.”
Detection of Phytophthora nicotianae and P. citrophthora in Citrus Roots and Soils by Nested PCR (2002, methodology): “Similar levels of sensitivity are reported for other phytopathogenic fungi (Faggian et al., 1999; Liew et al., 1998), and are essential to detect pathogens directly in soil or in infected tissues ( Schena et al., 2002b; Grote et al., 2002).”