Molecular maps of the reorganization of genome – nuclear lamina interactions during differentiation

By Daan Peric-Hupkes, W. Meuleman, Ludo Pagie, Sophia W. M. Bruggeman, Irina Solovei, W. Brugman, Stefan Gräf, Paul Flicek, R. Kerkhoven, M. van Lohuizen and 3 more. Molecules and Cells, 2010

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The three-dimensional organization of chromosomes within the nucleus and its dynamics during differentiation are largely unknown. To visualize this process in molecular detail, we generated high-resolution maps of genome – nuclear lamina interactions during subsequent differentiation of mouse embryonic stem cells via lineage-committed neural precursor cells into terminally differentiated astrocytes. This reveals that a basal chromosome architecture present in embryonic stem cells is cumulatively altered at hundreds of sites during lineage commitment and subsequent terminal differentiation. This remodeling involves both individual transcription units and multi-gene regions, and affects many genes that determine cellular identity. Often, genes that move away from the lamina are concomitantly activated; many others however remain inactive yet become unlocked for activation in a next differentiation step. These results suggest that lamina-genome interactions are widely involved in the control of gene expression programs during lineage commitment and terminal differentiation.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/f175e5ba1d41d06374471ff8b6ae59a90c6278c1

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Citations in the corpus

Step-wise methylation of histone H3K9 positions heterochromatin at the nuclear periphery. (2012, neutral): “Despite strong correlations between peripheral localization and transcriptional inactivity, the two are not strictly linked in worms or in mammals (Meister et al., 2010; Peric-Hupkes et al., 2010).”
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