Experimental annotation of the human genome using microarray technology

By D. Shoemaker, Eric E. Schadt, C. Armour, Yudong He, P. Garrett-engele, P. McDonagh, P. Loerch, A. Leonardson, P. Lum, G. Cavet and 25 more. Nature, 2001

469 citations recorded by the source corpus.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/f0bfd275c807074a369342005d797985831d15ba

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Citations in the corpus

Using the transcriptome to annotate the genome (2002, methodology): “Finally, LongSAGE data could be used to focus array-based gene verification strategie”
Expression profiling using microarrays fabricated by an ink-jet oligonucleotide synthesizer (2001, methodology): “We have also used this system to experimentally verify expression and structure of predicted genes across the entire human genom”
Navigating gene expression using microarrays — a technology review (2001, background): “The best example of this has been the use of the draft human genome sequence to design long oligonucleotides that correspond to predicted exon”
Applications of biotechnology for crop improvement: prospects and constraints (2002, background): “The last decade has seen the whole genome sequencing of model organisms such as human [5,6], yeast [7], Caenorhabditis elegans [8], Arabidopsis thaliana [9], and rice [10].”
A new approach to decoding life: systems biology. (2001, methodology): “GENETIC PARTS LIST Software and global experimental techniques are now becoming available to identify the gene locations, and even coding regions, embedded in a sequenced genome (110).”
The human genome browser at UCSC. (2002, methodology): “The first tracks incorporating DNA microarray data are the Rosetta tracks, which contain DNA probes for every predicted and confirmed exon on chromosome 22 as previously described (Shoemaker et al. 2001).”
Integrative Annotation of 21,037 Human Genes Validated by Full-Length cDNA Clones (2004, unknown): “The values obtained were smaller than those from other eukaryotes, and are inconsistent with estimates reported previously (Shoemaker et al. 2001).”
Normalization for cDNA microarray data: a robust composite method addressing single and multiple slide systematic variation. (2002, methodology): “Samples were purified and coupled to Cy3 or Cy5 as described (6,12,13).”
Widespread occurrence of antisense transcription in the human genome (2003, result): “This level of detection is comparable to that obtained in other studies, such as the 58% of known exons verified using microarray analysi”