Multisite phosphorylation of a CDK inhibitor sets a threshold for the onset of DNA replication

By Piers Nash, Xiaojing Tang, S. Orlicky, Qinghua Chen, F. Gertler, M. Mendenhall, F. Sicheri, Tony Pawson, Mike Tyers. Nature, 2001

831 citations recorded by the source corpus.

Subfields: Cell Biology, Molecular Biology, Oncology

View Paper: https://www.semanticscholar.org/paper/f0041274d580a6ff0c0bc35655ad49dfe6be95b6

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Cell Biology

In Molecular Biology

In Oncology

Citations in the corpus

Structure and function of WD40 domain proteins (2011, background): “Of the human 70 F-box proteins, about 20 of them contain the WD40 domain, which usually targets phosphorylated targets, such as Cyclin E (Hao et al., 2007), Catenin (Wu et al., 2003) and Sic1 ( Nash et al., 2001; Orlicky et al., 2003).”
Reading protein modifications with interaction domains (2006, background): “The requirement for multi-site phosphorylation of Sic1 sets a threshold for the level of G1 CDK activity that is necessary to promote Sic1 destruction, and it precipitates a switch-like entry into S phas”
RING domain E3 ubiquitin ligases. (2009, background): “By contrast, Pavletich’s group noted that Cdc4 has two basic pockets that bind the phosphates in the bisphosphorylated cyclin E degron (67).”
Transcriptional regulation by calcium, calcineurin, and NFAT. (2003, background): “Multisite phosphorylation is known to increase the sensitivity of biochemical processes to their signaling inputs (e.g., see Nash et al. 2001; Orlicky et al. 2003).”
Does Selection Mold Molecular Networks? (2003, background): “A single kinase, not an entire cascade, may suffice for switch-like activation of a target molecule if the target molecule has multiple sites that must all be phosphorylated for activation ( 5 ).”
Ubiquitin: structures, functions, mechanisms. (2004, background): “The properties of this regulatory modification are unusual: Sic1 must carry a large number of phosphates (five or six) before it can be recognized by Cdc4, but the phosphates can be distributed among any of nine potential sites [132,133].”
Biphasic Control of Microtubule Severing by Tubulin Glutamylation (2016, neutral): “The activation of the cyclindependent kinase inhibitor Sic1 is achieved via a sharp threshold response to multisite phosphorylation (Nash et al., 2001).”
How cells coordinate growth and division. (2004, background): “The multi-site phosphorylation of Whi5, and possibly Swi6, may make SBF/MBF activation ultrasensitive to Cln3–Cdc28 activity, thereby rendering Start entry ‘switch-like’ [76,86].”
Random-phase-approximation theory for sequence-dependent, biologically functional liquid-liquid phase separation of intrinsically disordered proteins (2016, methodology): “Here we examine computed variations in phase behavior with respect to block length and charge density of model polyampholytes of alternating equal-length charge blocks to gain insight into trends observed in IDP phase separation.”