Evaluation of the probes 2',7'-dichlorofluorescin diacetate, luminol, and lucigenin as indicators of reactive species formation.
By O. Myhre, J. M. Andersen, H. Aarnes, F. Fonnum. Biochemical Pharmacology, 2003
609 citations recorded by the source corpus.
Subfields: Immunology, Molecular Biology, Physiology
View Paper: https://www.semanticscholar.org/paper/e5fc490cd11f86f8e4c6d649498f8e3c22bd8e73
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In Immunology
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Citations in the corpus
- Fluorescence probes used for detection of reactive oxygen species. (2005, background): “The presence of cellular peroxidases is important for the oxidation of DCFH to DCF by H2O2 [38,41].”
- Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean? (2004, background): “Neither H2O2 nor O2 can oxidize DCFH, but peroxyl, alkoxyl, NO2 , carbonate (CO3 ) and OH radicals can, as can peroxynitrite (LeBel et al., 1992; Ischiropoulos et al., 1999; Bilski et al., 2002; Ohashi et al., 2002; Wardman et al., 2002; Myhre et al., 2003) (Table 5).”
- Oxidants, antioxidants and the ischemic brain (2004, background): “The brain has potent defenses against superoxide including dietary free-radical scavengers (ascorbate, α-tocopherol), the endogenous tripeptide glutathione, and enzymatic antioxidants.”
- Fluorescent and luminescent probes for measurement of oxidative and nitrosative species in cells and tissues: progress, pitfalls, and prospects. (2007, background): “Other catalysts reported having peroxidase-like activity with DCFH2 include free iron [91,113]; hemoglobin and myoglobin [98]; catalase [91,94]; Cu/Zn SOD [91,114–116]; xanthine oxidase [66,91,117]; lipoxygenase [91]; and prostaglandin H synthase [118].”
- Chemical and molecular mechanisms of antioxidants: experimental approaches and model systems (2009, background): “suggest that the DCF assay is only suitable for measurements of ONOO and H2O2 in combination with cellular peroxidases, peroxidases alone and ·OH, while it is not suitable for measurement of nitric oxide, HOCl or O2 • in biological systems [46].”
- Vapors Produced by Electronic Cigarettes and E-Juices with Flavorings Induce Toxicity, Oxidative Stress, and Inflammatory Response in Lung Epithelial Cells and in Mouse Lung (2015, methodology): “) from filtered research grade cigarettes (3R4F) was determined using 2’,7’dichlorofluorescein diacetate (H2 DCF-DA) fluorogenic probe (EMD Bioscience, CA) as described previously [18,19].”
- Hydrogen Peroxide: A Signaling Messenger (2006, neutral): “Unfortunately this compound does not react directly with H2O2 to any significant degree (147).”