Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean?

By Barry Halliwell, M. Whiteman. British Journal of Pharmacology, 2004

2,422 citations recorded by the source corpus.

Subfields: Biochemistry, Molecular Biology, Physiology

View Paper: https://www.semanticscholar.org/paper/be84b7ca863a75d9187a784cee351f31f46bc520

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Biochemistry

1. S. Biswas
2. Z. Ďuračková
3. P. Wardman
4. I. Dalle-Donne
5. A. Milzani
6. R. Rossi
7. R. Colombo
8. D. Giustarini

In Molecular Biology

1. S. Biswas
2. M. Murphy
3. Barry Halliwell
4. Z. Ďuračková
5. C. Winterbourn
6. P. Wardman
7. I. Dalle-Donne
8. A. Milzani

In Physiology

1. S. Biswas
2. C. Winterbourn
3. Barry Halliwell
4. P. Wardman
5. Z. Ďuračková
6. I. Dalle-Donne
7. A. Milzani
8. R. Rossi

Citations in the corpus

• Oxidative stress and neurodegeneration: where are we now? (2006, background): “Indeed, as we have learned to measure oxidative damage accurately, we find that it happens in all our tissues all the time, even under 21% O2; there is always a basal level of oxidative damage to DNA, lipids and proteins (reviewed by Halliwell and Whiteman 2004; Halliwell and Gutteridge 2006).”
• Impact of heat stress and nutritional interventions on poultry production (2018, background): “Oxidative stress reflects the imbalance between pro-oxidant and anti-oxidant systems, defined as the presence of reactive species, such as ROS, RNS and reactive chlorine species, in excess of the available antioxidant capacity of animal cells (Halliwell and Whiteman, 2004).”
• Some current insights into oxidative stress. (2010, background): “Br J Pharmacol 142:”
• Fluorescent and luminescent probes for measurement of oxidative and nitrosative species in cells and tissues: progress, pitfalls, and prospects. (2007, background): “, [5,75,77,91,96,97,307]), it is still appears not generally realized that the commonest probes for “reactive oxygen and nitrogen species” do not react directly at a significant rate with superoxide, hydrogen peroxide, peroxynitrite, or nitric oxide—although some individual studies, especially more ”
• Antioxidant response mechanism of freshwater microalgae species to reactive oxygen species production: a mini review (2020, background): “have shown that both the sugar and the base of DNA are prone to oxidation by hydroxyl radical, hydroxyl radical react with purine and pyrimidine resulting into products including 8-oxo-7,8 dehydro-2-deoxyguanosine, urea, hydroxymethyl urea, etc [16].”
• The antioxidant properties of serum albumin (2008, background): “Oxidative stress can result either from low levels of antioxidants and/or from an increased production of reactive species [2].”
• Reactive Species and Antioxidants. Redox Biology Is a Fundamental Theme of Aerobic Life (2006, background): “Indeed, as we learned how to measure oxidative damage (for review, see Halliwell and Whiteman, 2004), it was found to occur in aerobes even at normal O2 levels.”
• Unravelling the Biological Roles of Reactive Oxygen Species (2011, background): “Another consideration is that it is most unlikely that the biological mode of action of any antioxidant is through hydroxyl radical scavenging (Halliwell and Whiteman, 2004).”
• Biomarkers of oxidative damage in human disease. (2006, methodology): “Usually 8-hydroxy-20-deoxyguanosine (8OHdG) is measured as an index of oxidative DNA damage (7, 18, 152).”