A Low-Cost, High-Throughput Polyacrylamide Gel Electrophoresis System for Genotyping with Microsatellite DNA Markers

By Dechun Wang, Jian-rong Shi, S. Carlson, Perry B. Cregan, R. Ward, Brian W. Diers. 2003

217 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/e57f58593b9daaf247d4f09bd5065030e63c50a1

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In Plant Science

Citations in the corpus

Classification of North Dakota maize inbred lines into heterotic groups based on molecular and testcross data (2006, methodology): “Reaction products were electrophoresed on 6% polyacrylamide (19 acrylamide: 1 bis-acrylamide) gels and visualized by staining with ethidium bromide (Wang et al., 2003).”
Combining ability, genetic distances, and heterosis among Elite CIMMYT and IITA tropical maize inbred lines (2009, background): “DNA markers can now be used to assess the genetic divergence between germplasm pools and inbred lines with a reasonable degree of precision and cost (Warburton et al., 2002; Wang et al., 2003).”
Inheritance of Resistance to the Soybean Aphid in Soybean PI 200538 (2009, methodology): “:3 family in four-pot rows that 1196 WWW.CROPS.ORG CROP SCIENCE, VOL. 49, JULY–AUGUST 2009 Polymerase chain reaction (PCR) and evaluation of PCR products was performed as previously described (Wang et al., 2003).”
Genetic loci linking improved heat tolerance in wheat (Triticum aestivum L.) to lower leaf and spike temperatures under controlled conditions (2011, methodology): “Allele bands were visualized on 4% SFR agarose gels (Amresco) or 6% wide polyacrylamide gels ( Wang et al. 2003 ) depending on required band resolution and were visually scored.”
Fine mapping of a seed protein QTL on soybean linkage group I and its correlated effects on agronomic traits (2006, methodology): “The PCR products were analyzed by electrophoresis in 3% (w/v) Metaphor (FMC BioProducts, Rockland, ME) agarose gels, or in 6% (w/v) nondenaturing polyacrylamide gels and stained with ethidium bromide ( Wang et al., 2003 ).”
QTL associated with heat susceptibility index in wheat (Triticum aestivum L.) under short-term reproductive stage heat stress (2010, methodology): “Allele bands were visualized on 3% SFR agarose gels (Amresco, USA) or 6% wide polyacrylamide gels ( Wang et al. 2003 ) depending on required band resolution and were visually scored.”
Quantitative Trait Loci Contributing Resistance to Aflatoxin Accumulation in the Maize Inbred Mp313E (2005, methodology): “Analysis of variance of aflatoxin concentration in F2:3C.B.S. Scientific (Del Mar, CA) was used to obtain resolution families from the cross Mp313E B73 grown in four Missis-of polymorphisms as small as 2 bp (Wang et al., 2003).”
Development and mapping of microsatellite (SSR) markers in wheat (2005, methodology): “RIL banding patterns for BARC primer pairs were determined with the electrophoresis system described by Wang et al. (2003).”
Identification of QTLs Underlying Water-Logging Tolerance in Soybean (2005, methodology): “The PCR products were analyzed in 6% (w/v) non-denaturing polyacrylamide gel with a vertical sequencing system described by Wang et al. (2003).”
Analysis of Seed Zinc and Other Minerals in a Recombinant Inbred Population of Navy Bean (Phaseolus vulgaris L.) (2007, methodology): “The polymerase chain reaction was performed according to Blair et al. (2003), and DNA fragment analysis was performed using a high-throughput nondenaturing polyacrylamide gel electrophoresis system ( Wang et al., 2003 ).”
Bulked Segregant Analysis Using the GoldenGate Assay to Locate the Rpp3 Locus that Confers Resistance to Soybean Rust in Soybean (2009, methodology): “SSR allele size dif- ferences were determined as described by Wang et al. (2003) or with a 2% agarose gel.”
Mapping and confirmation of a new allele at Rpp1 from soybean PI 594538A conferring RB lesion-type resistance to soybean rust. (2009, methodology): “Polymerase chain reaction products were obtained for both nonlabeled and fl uorescently labeled SSR primers, followed by separation of nonlabeled products by nondenaturing polyacrylamide gel electrophoresis and for labeled products by ABI Prism 377 Genetic Analyzer (Applied Biosystems, Foster City, ”
Soybean aphid resistance genes in the soybean cultivars Dowling and Jackson map to linkage group M (2006, methodology): “PCR amplification conditions consisted of an initial denaturing step of 94� C for 4 min, followed by 34 cycles of: 25 s of denaturing at 94� C, 25 s of annealing at 47� C, and 25 s of extension at 68� C, with a final seven minute extension at 72� C. Gel electrophoresis was performed using non-denatu”
QTL associated with yield in three backcross-derived populations of soybean (2007, methodology): “ Wang et al. (2003) reported that the yield-increasing QTL allele originated from the domestic parent, and Smalley et al. (2004) noted that the QTL alleles associated with high yield were common to PIs and elite lines.”
Putative Alleles for Increased Yield from Soybean Plant Introductions (2004, methodology): “The nonlabeled PCR products multifactor ANOVA.were analyzed by electrophoresis in 6% nondenaturing poly- acrylamide gels (Sambrook et al., 1989; Wang et al., 2003) and stained with 1 g mL 1 ethidium bromide.”