Bulked Segregant Analysis Using the GoldenGate Assay to Locate the Rpp3 Locus that Confers Resistance to Soybean Rust in Soybean
By David L. Hyten, James R. Smith, R. Frederick, M. Tucker, Qijian Song, Perry B. Cregan. 2009
160 citations recorded by the source corpus.
Few resistance loci to soybean rust (SBR), caused by Phakopsora pachyrhizi Syd., have been genetically mapped and linked to molecular markers that can be used for marker assisted selection. New technologies are available for single nucleotide polymorphism (SNP) genotyping that can be used to rapidly map traits controlled by single loci such as resistance to SBR. Our objective was to demonstrate that the highthroughput SNP genotyping method known as the GoldenGate assay can be used to perform bulked segregant analysis (BSA) to fi nd candidate regions to facilitate effi cient mapping of a dominant resistant locus to SBR designated Rpp3. We used a 1536 SNP GoldenGate assay to perform BSA followed by simple sequence repeat (SSR) mapping in an F 2 population segregating for SBR resistance conditioned by Rpp3. A 13-cM region on linkage group C2 was the only candidate region identifi ed with BSA. Subsequent F 2 mapping placed Rpp3 between SSR markers BARC_Satt460 and BARC_Sat_263 on linkage group C2 which is the same region identifi ed by BSA. These results suggest that the GoldenGate assay was successful at implementing BSA, making it a powerful tool to quickly map qualitative traits since the GoldenGate assay is capable of screening 1536 SNPs on 192 DNA samples in three days.
Subfields: Molecular Biology, Plant Science
View Paper: https://www.semanticscholar.org/paper/9ce7b13391f1f53fe14ea0ff1df30b5a4d21d064
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In Molecular Biology
In Plant Science
- Brian W. Diers
- G. Hartman
- David L. Hyten
- R. Frederick
- Ellen D. West
- T. Herman
- Qijian Song
- Perry B. Cregan
Citations in the corpus
- A high density integrated genetic linkage map of soybean and the development of a 1536 universal soy linkage panel for quantitative trait locus mapping. (2010, methodology): “For example, the GoldenGate assay was coupled with bulked segregant analysis (BSA) to map the soybean rust resistance gene Rpp3 using SoyOPA-2 (Hyten et al., 2009).”
- Crops that feed the World 2. Soybean—worldwide production, use, and constraints caused by pathogens and pests (2011, background): “A number of recent studies mapped pathotype-specific genes for resistance to soybean rust to five loci (Garcia et al. 2008; Hyten et al. 2009; Hyten et al. 2007; Silva et al. 2008).”
- Mapping and confirmation of a new allele at Rpp1 from soybean PI 594538A conferring RB lesion-type resistance to soybean rust. (2009, background): “Therefore, rust-resistant cultivars are needed to reduce fungicide costs and yield losses due to SBR. Previous studies on host resistance to P. pachyrhizi have resulted in the identifi cation of the four dominant, independently inherited major genes Rpp1, Rpp2, Rpp3, and Rpp4 from PI 200492, PI 2309”