Development of Novel Fluorescence Probes That Can Reliably Detect Reactive Oxygen Species and Distinguish Specific Species* 210
By Ken-ichi Setsukinai, Y. Urano, K. Kakinuma, H. Majima, T. Nagano. Journal of Biological Chemistry, 2003
1,132 citations recorded by the source corpus.
We designed and synthesized 2-[6-(4′-hydroxy)phenoxy-3H-xanthen-3-on-9-yl]benzoic acid (HPF) and 2- [6-(4′-amino)phenoxy-3H-xanthen-3-on-9-yl]benzoic acid (APF) as novel fluorescence probes to detect selectively highly reactive oxygen species (hROS) such as hydroxyl radical (⋅OH) and reactive intermediates of peroxidase. Although HPF and APF themselves scarcely fluoresced, APF selectively and dose-dependently afforded a strongly fluorescent compound, fluorescein, upon reaction with hROS and hypochlorite (−OCl), but not other reactive oxygen species (ROS). HPF similarly afforded fluorescein upon reaction with hROS only. Therefore, not only can hROS be differentiated from hydrogen peroxide (H2O2), nitric oxide (NO), and superoxide (O 2 ⨪ ) by using HPF or APF alone, but −OCl can also be specifically detected by using HPF and APF together. Furthermore, we applied HPF and APF to living cells and found that HPF and APF were resistant to light-induced autoxidation, unlike 2′,7′-dichlorodihydrofluorescein, and for the first time we could visualize −OCl generated in stimulated neutrophils. HPF and APF should be useful as tools to study the roles of hROS and−OCl in many biological and chemical applications.
Subfields: Bioengineering, Electrical and Electronic Engineering, Physiology
View Paper: https://www.semanticscholar.org/paper/e487a35403e64aa2eb9d14f63de9ca33340e6722
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Citations in the corpus
- Sub-lethal antibiotic treatment leads to multidrug resistance via radical-induced mutagenesis (2010, methodology): “Thiourea is a potent hydroxyl radical scavenger that mitigates the effects of hydroxyl radical damage in both prokaryotes and eukaryotes (Novogrodsky et al., 1982; Repine fold change mutation rate % increase HPF signal R 2 = 0.8455”
- Fluorescence probes used for detection of reactive oxygen species. (2005, background): “[100] studied the reactivity of these two probes for several ROS and RNS.”
- ROS-dependent activation of the TRAF6-ASK1-p38 pathway is selectively required for TLR4-mediated innate immunity (2005, methodology): “For the detection of ROS, the cells were mixed with 20 μM hydroxyphenyl fluorescein (HPF”
- Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals (2007, background): “In contrast, H 2 treatment significantly decreased levels of •OH, as assessed by the fluorescence signal emitted by the oxidized form of 2-[6-(4′-hydroxy)phenoxy-3 H -xanthen-3-on-9-yl] benzoate (HPF) (refs”
- A common mechanism of cellular death induced by bactericidal antibiotics. (2007, positive): “Using the dye hydroxyphenyl fluorescein (HPF), which is oxidized by hydroxyl radicals with high specificity ( Setsukinai et al., 2003 ), we first examined a concentration of hydrogen peroxide known to induce hydroxyl radical formation via Fenton chemistry (Imlay et al., 1988).”
- Silver Enhances Antibiotic Activity Against Gram-negative Bacteria (2013, methodology): “coli cells and in cells treated with Ag for 1 hour using 3′-(p-hydroxyphenyl) fluorescein (HPF) dye (24).”
- Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean? (2004, methodology): “Let us now comment on the use of probes, usually fluorescence based, that are alleged to detect cellular produc- tion of RS. Dichlorofluorescin diacetate (DCFDA) DCFDA is the most popular of these probes, frequently being used to detect ‘cellular peroxides’.”
- Antibiotics induce redox-related physiological alterations as part of their lethality (2014, methodology): “We previously have used 3′-(p-hydroxyphenyl) fluorescein (HPF), which has high reported in vitro selectivity for highly reactive species which include hydroxyl radicals but not superoxide or H2O2 (52), to assess antibiotic-induced ROS production in both Gram-negative and Gram-positive bacteria (7, 8”