Regulation of Brassinosteroid Signaling by a GSK3/SHAGGY-Like Kinase

By Jianming Li, K. Nam. Science, 2002

636 citations recorded by the source corpus.

GSK3/SHAGGY is a highly conserved serine/threonine kinase implicated in many signaling pathways in eukaryotes. Although many GSK3/SHAGGY-like kinases have been identified in plants, little is known about their functions in plant growth and development. Here we show that the Arabidopsis BRASSINOSTEROID-INSENSITIVE 2(BIN2) gene encodes a GSK3/SHAGGY-like kinase. Gain-of-function mutations within its coding sequence or its overexpression inhibit brassinosteroid (BR) signaling, resulting in plants that resemble BR-deficient and BR-response mutants. In contrast, reduced BIN2 expression via cosuppression partially rescues a weak BR-signaling mutation. Thus, BIN2 acts as a negative regulator to control steroid signaling in plants.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/e3f9d70cee84122caf34489cea8c2e17f30f17c8

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

1. Zhi-Yong Wang
2. Tae-Wuk Kim
3. Yanhai Yin
4. Joanne Chory
5. S. Yoshida
6. T. Asami
7. Meng Chen
8. Todd C. Mockler

In Plant Science

1. Zhi-Yong Wang
2. Tae-Wuk Kim
3. Yanhai Yin
4. Joanne Chory
5. S. Yoshida
6. Rajendra Bari
7. T. Asami
8. Todd C. Mockler

Citations in the corpus

• BES1 accumulates in the nucleus in response to brassinosteroids to regulate gene expression and promote stem elongation. (2002, positive): “In addition to the sequence homology between BES1 cDNA was amplified by RT-PCR using first strand cDNA generBIN2 and GSK-3 (Li and Nam, 2002), their corresponding ated from wild-type Col RNA using Pfu DNA polymerase (Strasubstrates BES1 and -catenin are both negatively regu- tagene).”
• A brassinosteroid transcriptional network revealed by genome-wide identification of BESI target genes in Arabidopsis thaliana. (2011, neutral): “signaling (Choe et al., 2002; Li and Nam, 2002; Pérez-Pérez et al., 2002; Peng et al., 2010).”
• Role of plant hormones in plant defence responses (2009, background): “BIN2 is one of the 10 GSK3-like kinases in Arabidopsis (Jonak and Hirt 2002) and is considered to be a negative regulator of the brassinosteroid signal transduction pathway (Li and Nam 2002).”
• Interdependency of Brassinosteroid and Auxin Signaling in Arabidopsis (2004, background): “A Shaggy/GSK3-type kinase, Brassinosteroid Insensitive-2 (BIN2), acts as a negative regulator of the pathway downstream of BRI1 action (Li and Nam 2002).”
• Brassinosteroids: Multidimensional Regulators of Plant Growth, Development, and Stress Responses[OPEN] (2019, background): “Lastly, BES1 and BZR1 are regulated by multiple E3 ubiquitin ligases that control their degradation through the26Sproteasome or selective autophagy.”
• Nuclear-localized BZR1 mediates brassinosteroid-induced growth and feedback suppression of brassinosteroid biosynthesis. (2002, background): “Recent molecular genetic studies Plant steroid hormones, brassinosteroids (BRs), are perceived by a cell surface receptor kinase, BRI1, but have implicated a glycogen synthase kinase 3-like protein (BIN2) as a negative regulator of BR responses (Li how BR binding leads to regulation of gene expressi”
• Brassinosteroid signal transduction from receptor kinases to transcription factors. (2010, background): “BR INSENSITIVE 1 (BRI1) encodes one of over 200 members of the leucine-rich repeat-receptor-like kinase (LRR-RLK) family in Arabidopsis (79), and BR INSENSITIVE 2 (BIN2) encodes one of the 10 glycogen synthase kinase 3 (GSK3)-like kinases of Arabidopsis (50).”
• Brassinosteroid signal transduction from cell surface receptor kinases to nuclear transcription factors (2009, background): “Downstream BR signalling involves the GSK3-like kinase BIN2 (ref”