Brassinosteroid signal transduction from cell surface receptor kinases to nuclear transcription factors
By Tae-Wuk Kim, S. Guan, Yu Sun, Zhiping Deng, Wenqiang Tang, Jianxiu Shang, Ying Sun, Alma Burlingame, Zhi-Yong Wang. Nature Cell Biology, 2009
650 citations recorded by the source corpus.
Brassinosteroid (BR) regulates gene expression and plant development through a receptor kinase-mediated signal transduction pathway. Despite the identification of many components of this pathway, it remains unclear how the BR signal is transduced from the cell surface to the nucleus. Here we describe a complete BR signalling pathway by elucidating key missing steps. We show that phosphorylation of BSK1 (BR-signalling kinase 1) by the BR receptor kinase BRI1 (BR-insensitive 1) promotes BSK1 binding to the BSU1 (BRI1 suppressor 1) phosphatase, and BSU1 inactivates the GSK3-like kinase BIN2 (BR-insensitive 2) by dephosphorylating a conserved phospho-tyrosine residue (pTyr 200). Mutations that affect phosphorylation/dephosphorylation of BIN2 pTyr200 (bin2-1, bin2-Y200F and quadruple loss-of-function of BSU1-related phosphatases) support an essential role for BSU1-mediated BIN2 dephosphorylation in BR-dependent plant growth. These results demonstrate direct sequential BR activation of BRI1, BSK1 and BSU1, and inactivation of BIN2, leading to accumulation of unphosphorylated BZR (brassinazole resistant) transcription factors in the nucleus. This study establishes a fully connected BR signalling pathway and provides new insights into the mechanism of GSK3 regulation.
Subfields: Molecular Biology, Plant Science
View Paper: https://www.semanticscholar.org/paper/3130ac1c8105b867df112a22b767385f6e2bd9ce
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Citations in the corpus
- Integration of Brassinosteroid Signal Transduction with the Transcription Network for Plant Growth Regulation in Arabidopsis (2010, positive): “BRs act through the BRI1 receptor-like kinase and its well-defined signal transduction pathway to activate members of the BZR family transcription factors (Kim et al., 2009).”
- Interplay between reactive oxygen species and hormones in the control of plant development and stress tolerance. (2015, background): “ABA promotes phosphorylation of BES1 in the presence of BR through activation of BIN2 (S. Zhang et al., 2009).”
- Brassinosteroid signal transduction from receptor kinases to transcription factors. (2010, background): “However, as a negative regulator of BR signaling, BIN2 is inactivated by BR (42, 65), which would lead to BR activation of ARF2.”