Interdependency of Brassinosteroid and Auxin Signaling in Arabidopsis

By Jennifer L. Nemhauser, Todd C. Mockler, Joanne Chory. PLoS Biology, 2004

599 citations recorded by the source corpus.

How growth regulators provoke context-specific signals is a fundamental question in developmental biology. In plants, both auxin and brassinosteroids (BRs) promote cell expansion, and it was thought that they activated this process through independent mechanisms. In this work, we describe a shared auxin:BR pathway required for seedling growth. Genetic, physiological, and genomic analyses demonstrate that response from one pathway requires the function of the other, and that this interdependence does not act at the level of hormone biosynthetic control. Increased auxin levels saturate the BR-stimulated growth response and greatly reduce BR effects on gene expression. Integration of these two pathways is downstream from BES1 and Aux/IAA proteins, the last known regulatory factors acting downstream of each hormone, and is likely to occur directly on the promoters of auxin:BR target genes. We have developed a new approach to identify potential regulatory elements acting in each hormone pathway, as well as in the shared auxin:BR pathway. We show that one element highly overrepresented in the promoters of auxin- and BR-induced genes is responsive to both hormones and requires BR biosynthesis for normal expression. This work fundamentally alters our view of BR and auxin signaling and describes a powerful new approach to identify regulatory elements required for response to specific stimuli.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/6e69c8b8cdaef12ef26a5936c7458e42e4db1fde

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Citations in the corpus

The AtGenExpress hormone and chemical treatment data set: experimental design, data evaluation, model data analysis and data access. (2008, methodology): “Nemhauser et al. (2006) used our data to analyze hormone networks from the point of view of hormone metabolism.”
Auxin receptors and plant development: a new signaling paradigm. (2008, background): “Recent studies either have suggested (Nemhauser et al. 2004) or have demonstrated (Shin et al. 2007) that unrelated transcription factors act within or near ARF complexes.”
Plant hormones are versatile chemical regulators of plant growth. (2009, background): “Further, the promoters of many auxin response genes don't have an AuxRE , which suggests that other DNA sequences are probably involved in ARF functio”
Auxin control of root development. (2010, background): “For instance, there is accumulating evidence for a rate-limiting role of the brassinosteroid pathway in the auxin response (Nemhauser et al. 2004; Hardtke et al. 2007; Kuppusamy et al. 2008; Vert et al. 2008).”
A new class of transcription factors mediates brassinosteroid-regulated gene expression in Arabidopsis. (2005, unknown): “Deletion of the BIN2 phosphorylation sites (P) stabilizes the pro- sequences are enriched in many BR-induced gene promoters (Nemhauser et al., 2004), suggesting that BES1 tein and leads to a bes1-D phenotype (Figures 2C and 2D), providing direct evidence that BIN2 phosphoryla- may directly bind to s”
Brassinosteroids and their role in response of plants to abiotic stresses (2014, background): “Clouse and Sasse (1998) and Sasse (2003) disclosed that BRs are required for normal growth and development including shoot and root growth (Nemhauser et al. 2004), vascular differentiation (Caño-Delgado et al.”
Integration of Brassinosteroid Signal Transduction with the Transcription Network for Plant Growth Regulation in Arabidopsis (2010, unknown): “Functional interactions between BR and other hormones have been observed at the physiological and gene expression levels (Gendron et al., 2008; Goda et al., 2004; Hardtke et al., 2007; Nemhauser et al., 2004).”
Interplay between reactive oxygen species and hormones in the control of plant development and stress tolerance. (2015, background): “Brassinosteroids (BRs) function in conjunction with auxin to enhance growth and regulate gene expression in a synergistic manner (Nemhauser et al., 2004).”
Regulatory Components of Shade Avoidance Syndrome (2010, unknown): “elements (Nemhauser et al., 2004).”
A brassinosteroid transcriptional network revealed by genome-wide identification of BESI target genes in Arabidopsis thaliana. (2011, background): “We first compared the 1609 BES1 targets identified by ChIPchip with 2163 BR-regulated genes (Tables S2 and S3) at different developmental stages, including the ones from this study (see next) and those published previously (Goda et al., 2004; Nemhauser et al., 2004; Guo et al., 2009).”
Auxin: regulation, action, and interaction. (2005, background): “More recently, genome-wide profiling experiments have revealed a wealth of auxin-induced genes (Sawa et al., 2002; Pufky et al., 2003; Cluis et al., 2004; Himanen et al., 2004), many of which contain AuxREs in putative regulatory regions (Pufky et al., 2003; Nemhauser et al., 2004).”