Correction of multi-gene deficiency in vivo using a single 'self-cleaving' 2A peptide–based retroviral vector

By Andrea L. Szymczak, C. Workman, Yao Wang, Kate M. Vignali, S. Dilioglou, E. Vanin, D. Vignali. Nature Biotechnology, 2004

1,226 citations recorded by the source corpus.

Subfields: Cardiology and Cardiovascular Medicine, Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/debcf986c6f5df8b1af954b9d8ee546816e666d8

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In Genetics

1. José C. R. Silva
2. Jesús Gil
3. G. Feng
4. L. Vallier
5. Michel Sadelain

In Molecular Biology

1. José C. R. Silva
2. Jesús Gil
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Citations in the corpus

• Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair (2014, methodology): “This reading frame was preceded by an E2A peptide linker for multicistronic expression (Fig. 1A; Szymczak et al. 2004).”
• Stoichiometric and temporal requirements of Oct4, Sox2, Klf4, and c-Myc expression for efficient human iPSC induction and differentiation (2009, methodology): “Overlapping PCR was used to generate bicistronic expression cassettes encoding vexGFP, mCitrine, mCherry, and mCerulean linked by a P2A peptide preceded by a Gly-Ser-Gly linker (35) to the cDNAs of human Oct4, Sox2, Klf4 and c-Myc, respectively, which were cloned in a lentiviral vector under the tra”
• Generation of transgene-free induced pluripotent mouse stem cells by the piggyBac transposon (2009, neutral): “The approximately 20–amino acid 2A peptides from foot-and-mouth disease virus (F2A) and Thosea asigna virus (T2A) work as self-cleaving signals and enable expression of several gene products from a single transcrip”
• Senescence impairs successful reprogramming to pluripotent stem cells. (2009, methodology): “which are 18 to 22 amino acids long have been used to express multiple proteins from a single transcript, by mediating “ribosomal skipping” (Doronina et al., 2008; Ryan and Drew, 1994; Szymczak et al., 2004).”
• Genetic dissection of neural circuits. (2008, neutral): “Another promising strategy is to link the open reading frames of the endogenous and target genes with the self-cleaving 2A peptide; the selfcleavage of the peptide results in equal expression of two proteins (e.g., Szymczak et al., 2004).”
• High Cleavage Efficiency of a 2A Peptide Derived from Porcine Teschovirus-1 in Human Cell Lines, Zebrafish and Mice (2011, methodology): “the N-terminus of a 2A peptide can improve cleavage efficiency [11,18].”
• Imaging Neural Activity Using Thy1-GCaMP Transgenic mice (2012, methodology): “2c and GCaMP3, we coexpressed GCaMPs and the red fluorescence protein tdTomato in the same construct using the 2A peptide (P2A) sequence (Szymczak et al., 2004) in HEK293 cells.”